{"product_id":"ucf-me-ultranuclease-gmp-grade-250-u-mu-l-bhz20800050","title":"UCF.ME™ UltraNuclease GMP-grade (250 U\/μL)","description":"\u003cp style=\"font-weight: 400;\"\u003eUltraNuclease is a genetically engineered endonuclease from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eSerratia marcescens. \u003c\/em\u003eThe enzyme digests all forms of DNA\/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein\/polysaccharide pharmaceutical manufacturing.  It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eUltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eWide range of applications: degrade all forms of DNA and RNA \u003c\/li\u003e\n\u003cli\u003eHigh purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eU\/mg\u003c\/li\u003e\n\u003cli\u003eStrong adaptability: strong tolerance, adapt to a variety of reaction conditions\u003c\/li\u003e\n\u003cli\u003eComply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin\u003c\/li\u003e\n\u003cli\u003eQualification: product gets the FDA DMF filling which can shorten the customers for new drug application\u003c\/li\u003e\n\u003cli\u003eGMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003ePurification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA\/RNA from proteins and other biologicals\u003c\/li\u003e\n\u003cli\u003eReduction of viscosity caused by nucleic acids\u003c\/li\u003e\n\u003cli\u003eSample preparation in electrophoresis and chromatography\u003c\/li\u003e\n\u003cli\u003ePrevention of cell clumping\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable height=\"257\" style=\"width: 812px; height: 245px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003eRecombinant E. coli with UltraNuclease gene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e26.5 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003eIsoelectric point\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e6.85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 24.5052px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 24.5052px;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 24.5052px;\"\u003e≥ 99% (SDS-PAGE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 27.9818px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 27.9818px;\"\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 27.9818px;\"\u003e20 mM Tris-HCl pH8.0, 2 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 20 mM NaCl, 50% glycerin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 95.7812px;\"\u003e\n\u003ctd style=\"width: 160.326px; height: 95.7812px;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"width: 637.708px; height: 95.7812px;\"\u003eThe definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 100.027%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.2804%;\"\u003eComponents No.\u003c\/td\u003e\n\u003ctd style=\"width: 18.4883%;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES25 (25 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 15.2176%;\"\u003e20157ES60 (100 KU)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES80 (1 MU)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20157ES90 (5 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.2804%;\"\u003e20157\u003c\/td\u003e\n\u003ctd style=\"width: 18.4883%;\"\u003eUltraNuclease GMP-grade (250 U\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 15.2176%;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.8553%;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eQuality\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNo.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eTest Items\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eCompetitor\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e Yeasen\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eE. coli\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eE. coli\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eActivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 250 units\/μL \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e250-300 units\/μL \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e3\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecific Activity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥1.1×10\u003csup\u003e6\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003eU\/mg\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥1.5×10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eU\/mg\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e4\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 99%（SDS）\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e≥ 99%（HPLC）\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e\u0026lt; 0.25EU\/1000U\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e\u0026lt; 0.25EU\/1000U\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003e6\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eProtease\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNone Detected\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNone Detected\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cul\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eUltraNuclease \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eEnzymatic \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eActivity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e \u003cspan style=\"font-family: Inter Medium;\"\u003eUnder Different Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eTemperature \u0026amp; pH\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 1. Effects of temperature and pH on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_f3e27588-04cc-429b-af21-66c8e25bfd62_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 1. Effects of temperature and pH on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e(A) Enzyme activity increases with temperature, with the optimum at 37 °C.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e(B) The enzyme is active within pH 4–10, with pH 8.0 as the optimum.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eSalt ion concentration\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 2. Effects of salt ion concentration on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_b380caa3-f787-416c-a1fe-c67e9fdb42df_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eFigure \u003c\/span\u003e2\u003cspan class=\"15\"\u003e. Effects of salt ion concentration on enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(A). Na⁺\/K⁺ inhibit enzyme activity; activity is completely lost above 300 mM, while good activity is retained below 20 mM.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(B). Maximum activity is observed with 5 mM Mg²⁺; in the absence of Mg²⁺, 1–2 mM Mn²⁺ can support activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(C). Increasing PO₄³⁻ concentration significantly inhibits enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eSurfactants\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 3. Effects of surfactants on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_9203e49b-bc65-4eaf-b571-73449d80442d_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 3. Effects of surfactants on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e(A) Triton® X-100 and (B) sodium deoxycholate reduce enzyme activity and should be maintained at low concentrations.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eProtein denaturants\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 4. Effect of protein denaturants on enzyme activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_05d55a6a-9393-4b2e-b737-f31479947c4c_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 4. Effect of protein denaturants on enzyme activity.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eSDS and urea reduce enzyme activity and should be maintained at low concentrations.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eOther commonly used reagents\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 5. Effects of different inhibitors on UltraNuclease activity.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_c0ddd62f-a3fa-4e0f-b1db-42ada7393697_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003eFigure\u003c\/span\u003e 5\u003cspan class=\"15\"\u003e. Effects of different inhibitors on UltraNuclease activity.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eA\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. Enzyme activity is strongly influenced by ammonium sulfate concentration.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eB\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. PMSF shows little effect on enzyme activity.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eC\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e. At low EDTA concentrations (≤0.5 mM), enzyme activity is largely unaffected, whereas at 5 mM EDTA, activity is completely inhibited.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_7ffd81d4-9f52-4bad-afc1-93f728c332b7_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cul\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eRobust Performance\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 6. Single-factor test comparison with Supplier M*.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_899fdedb-9633-4060-99d0-10ff8921c18d_1024x1024.png?v=1757913170\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 6. Single-factor test comparison with Supplier M*.\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eNo significant difference was observed between Yeasen UltraNuclease and supplier M*.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cstrong\u003eStrong stability\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 7. Stability of UltraNuclease.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/8_72f7094f-ba1f-4f6f-b950-f4ff92ff503f_1024x1024.png?v=1757913475\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003eFigure 7. Stability of UltraNuclease.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan class=\"15\"\u003e(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eReaction conditions recommend\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cstrong\u003eTable 1. The optimal reaction conditions for UCF.ME™ UltraNuclease\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFactor\u003c\/td\u003e\n\u003ctd\u003eOptimum Condition\u003c\/td\u003e\n\u003ctd\u003eEffective condition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMg\u003csup\u003e2+\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e1-5mM\u003c\/td\u003e\n\u003ctd\u003e0-10mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH\u003c\/td\u003e\n\u003ctd\u003e8-9\u003c\/td\u003e\n\u003ctd\u003e6-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTemperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003ctd\u003e0-42℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNa+\/K+\u003c\/td\u003e\n\u003ctd\u003e0-20mM\u003c\/td\u003e\n\u003ctd\u003e0-150mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePO\u003csub\u003e4\u003c\/sub\u003e\u003csup\u003e3+\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e0-10mM\u003c\/td\u003e\n\u003ctd\u003e0-100mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA\u003c\/td\u003e\n\u003ctd\u003e0-0.5mM\u003c\/td\u003e\n\u003ctd\u003e0-5mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTriton®X-100\u003c\/td\u003e\n\u003ctd\u003e0%-0.4%\u003c\/td\u003e\n\u003ctd\u003e0%-1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"25 KU","offer_id":53294149337453,"sku":"20157ES25","price":155.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294159266157,"sku":"20157ES60","price":355.0,"currency_code":"USD","in_stock":true},{"title":"1 MU (1000 KU)","offer_id":53294159298925,"sku":"20157ES80","price":1590.0,"currency_code":"USD","in_stock":true},{"title":"5 MU (5000 KU)","offer_id":53294159331693,"sku":"20157ES90","price":3085.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_194d99bf-687a-4a20-993d-99e720b68522.png?v=1778101064","url":"https:\/\/www.ebiohippo.com\/products\/ucf-me-ultranuclease-gmp-grade-250-u-mu-l-bhz20800050","provider":"BioHippo","version":"1.0","type":"link"}