{"product_id":"ultra-pure-phi29-dna-polymerase-bhz10900048","title":"Ultra-Pure Phi29 DNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003eUltra-Pure Phi29 DNA Polymerase\u003c\/strong\u003e is a highly \u003cstrong\u003eprocessive\u003c\/strong\u003e enzyme known for its powerful \u003cstrong\u003estrand-displacement activity\u003c\/strong\u003e, making it ideal for \u003cstrong\u003eisothermal amplification\u003c\/strong\u003e of both circular and linear DNA templates. It is particularly effective in techniques such as \u003cstrong\u003eRolling Circle Amplification (RCA)\u003c\/strong\u003e, \u003cstrong\u003eMultiple Displacement Amplification (MDA)\u003c\/strong\u003e, and \u003cstrong\u003eWhole Genome Amplification (WGA)\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eThanks to its \u003cstrong\u003e3'→5' exonuclease activity\u003c\/strong\u003e, Ultra-Pure Phi29 DNA Polymerase boasts \u003cstrong\u003eexceptional fidelity\u003c\/strong\u003e, enabling the amplification of even \u003cstrong\u003every small quantities of DNA templates\u003c\/strong\u003e. The enzyme undergoes an advanced, multi-step purification process that utilizes a combination of \u003cstrong\u003ephysical, chemical, and enzymatic methods\u003c\/strong\u003e to ensure the complete removal of contaminating genomic DNA. Rigorous \u003cstrong\u003equality control\u003c\/strong\u003e is performed, including a \u003cstrong\u003enon-specific DNase Activity Assay\u003c\/strong\u003e and a \u003cstrong\u003eqPCR DNA Contamination Test\u003c\/strong\u003e using a TaqMan probe, ensuring superior purity and performance.\u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin-top: 30px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eProduct Component\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003eUltra-Pure Phi29 DNA Polymerase\u003c\/td\u003e\n\u003ctd\u003e100 μl (200 rxn)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5X Ultra-Pure Phi29 DNA Polymerase Reaction Buffer\u003csup\u003e1\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd\u003e500 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 10pt;\"\u003e\u003csup\u003e1\u003c\/sup\u003eBuffer contains 0.5 μg\/μl BSA. Keep at -20°C at all times except when using the product.\u003c\/span\u003e\u003c\/p\u003e\u003ctable class=\"bhc-specs-table\" style=\"width:100%;border-collapse:collapse\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#1a5c58;color:#fff\"\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eEnzyme Type\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eDNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eEnzyme Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003e3'→5' Proofreading\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eStore all components at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 ul","offer_id":53294164083053,"sku":"E014","price":157.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/l4omThKmjrvxxMY6LuX0fC7nkK7pCW2wPZ6liIIs.png?v=1778485104","url":"https:\/\/www.ebiohippo.com\/products\/ultra-pure-phi29-dna-polymerase-bhz10900048","provider":"BioHippo","version":"1.0","type":"link"}