{"product_id":"upf1-antibody-up-frameshift-suppressor-1-rent1-bha17136365","title":"UPF1 Antibody \/ Up-frameshift suppressor 1 \/ RENT1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eUPF1 Antibody \/ Up-frameshift suppressor 1 \/ RENT1 is a anti-UPF1 Mouse antibody Recombinant Rabbit Monoclonal clone 19U80 supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e UPF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Mouse, Recombinant Rabbit Monoclonal, clone 19U80, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eUPF1 antibody is a central reagent for research on RNA surveillance, gene regulation, and cellular quality control. The encoded protein, up frameshift suppressor 1 (UPF1), is an RNA helicase and ATPase that plays an essential role in nonsense mediated mRNA decay (NMD). This pathway detects and degrades mRNAs containing premature stop codons, preventing the accumulation of truncated proteins that could be harmful to cells. Through this activity, UPF1 safeguards transcriptome integrity and helps maintain proper gene expression.\u003cbr\u003e\u003cbr\u003eUPF1 associates with the exon junction complex and interacts with other NMD factors, including UPF2 and UPF3, to form surveillance complexes on target transcripts. Its helicase activity enables it to remodel RNA protein complexes, while its phosphorylation state determines whether bound transcripts are degraded or stabilized. Beyond NMD, UPF1 has also been implicated in telomere maintenance, cell cycle regulation, and DNA replication stress responses, underscoring its multifunctional nature.\u003cbr\u003e\u003cbr\u003eResearch has linked UPF1 dysregulation to human disease. Mutations or altered expression of UPF1 can disrupt RNA surveillance, contributing to neurodevelopmental disorders and cancer. For example, reduced NMD activity has been associated with enhanced tumor progression due to the stabilization of aberrant mRNAs. Conversely, hyperactive NMD can impair expression of regulatory transcripts, leading to developmental abnormalities. These findings highlight UPF1 as a key factor in both normal physiology and disease pathology.\u003cbr\u003e\u003cbr\u003eAt the molecular level, UPF1 contains an RNA helicase domain with ATP binding motifs and regulatory regions that mediate interactions with NMD cofactors. Phosphorylation of UPF1 by SMG1 kinase is a crucial step in triggering mRNA decay. Structural and biochemical studies have shown that UPF1 undergoes major conformational changes upon RNA binding and phosphorylation, enabling it to act as a dynamic regulator of RNA fate.\u003cbr\u003e\u003cbr\u003eThe UPF1 antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect protein expression, localization, and phosphorylation status. These applications support research into RNA decay, transcriptional fidelity, and cancer biology. For investigators studying RNA metabolism, gene expression control, or surveillance pathways, the UPF1 antibody is a reliable and specific detection tool.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=UPF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=UPF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=UPF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/UPF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=UPF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ug","offer_id":53047326769517,"sku":"RQ8947","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b68428c5-7d73-439c-b02f-12a15380335c.jpg?v=1772019450","url":"https:\/\/www.ebiohippo.com\/products\/upf1-antibody-up-frameshift-suppressor-1-rent1-bha17136365","provider":"BioHippo","version":"1.0","type":"link"}