{"product_id":"upre-reporter-lentivirus-bhv19400032","title":"UPRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe unfolded protein response (UPR) is an adaptive signaling network that maintains protein-folding homeostasis in the endoplasmic reticulum. When misfolded proteins accumulate, three ER sensors are activated: IRE1 (ERN1), which splices XBP1 mRNA to generate the active transcription factor sXBP1; ATF6, which is cleaved to release a transcriptionally active fragment; and PERK. The unfolded protein response element (UPRE) is a promoter motif bound by sXBP1 and ATF6 to induce genes encoding chaperones and components of ER-associated degradation. Sustained or unresolved ER stress contributes to metabolic disease, neurodegeneration, and cancer, making UPRE-dependent transcription a key readout of cellular stress signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe UPRE Reporter Lentivirus is a transcription-factor reporter system that detects unfolded protein response activity in human and mouse cells. The construct uses tandem repeats of the UPR element, containing DNA-binding motifs for sXBP1 and ATF6, to drive a fluorescent or luminescent reporter (firefly luciferase, Renilla luciferase, GFP, or RFP). A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe system is suited to monitoring ER stress, studying IRE1-XBP1 and ATF6 signaling, and screening compounds that modulate the UPR. Particles are purified by PEG precipitation and sucrose gradient centrifugation, enabling effective transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Unfolded protein response (UPR) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625877869,"sku":"LTV-0036-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896144749,"sku":"LTV-0036-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896177517,"sku":"LTV-0036-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896210285,"sku":"LTV-0036-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896243053,"sku":"LTV-0036-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896275821,"sku":"LTV-0036-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896308589,"sku":"LTV-0036-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_0c83e104-b89b-4f37-8b60-4ca6b58449ac.jpg?v=1776934147","url":"https:\/\/www.ebiohippo.com\/products\/upre-reporter-lentivirus-bhv19400032","provider":"BioHippo","version":"1.0","type":"link"}