{"product_id":"usp4-antibody-hrp-conjugated-bha10531785","title":"USP4 Antibody, HRP conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-USP4 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect USP4 in Human for cell biology research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Ubiquitin carboxyl-terminal hydrolase 4 protein (632-810AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHRP:\u003c\/strong\u003e Direct detection (TMB\/ECL); quench endogenous peroxidase in tissue assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eUSP4\u003c\/strong\u003e (also: Deubiquitinating enzyme 4, MGC149848, MGC149849, Proto oncogene, Protooncogene, Ubiquitin carboxyl terminal hydrolase 4, Ubiquitin carboxyl-terminal hydrolase 4, Ubiquitin specific peptidase 4 (proto oncogene), Ubiquitin specific peptidase 4 (protooncogene), Ubiquitin specific peptidase 4, Ubiquitin specific processing protease 4, Ubiquitin specific protease 4 (proto oncogene), Ubiquitin specific protease 4 (protooncogene), Ubiquitin specific protease 4, Ubiquitin specific protease proto oncogene, Ubiquitin specific protease protooncogene, Ubiquitin thioesterase 4, Ubiquitin thiolesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, UBP4_HUMAN, UNP, UNPH, USP 4, USP4) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current USP4 annotation relevant to cell biology.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241115476333,"sku":"CSB-PA614393LB01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241206407533,"sku":"CSB-PA614393LB01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_e06c48d1-c360-4b09-b24d-5b5021e7fd30.jpg?v=1784927064","url":"https:\/\/www.ebiohippo.com\/products\/usp4-antibody-hrp-conjugated-bha10531785","provider":"BioHippo","version":"1.0","type":"link"}