{"product_id":"usp5-antibody-ubiquitin-specific-peptidase-5-bha17135409","title":"USP5 Antibody \/ Ubiquitin specific peptidase 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eUSP5 Antibody \/ Ubiquitin specific peptidase 5 is a anti-USP5 Rabbit antibody Recombinant Rabbit Monoclonal clone 32U18 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e USP5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32U18, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eUSP5 antibody detects ubiquitin specific peptidase 5, a deubiquitinating enzyme encoded by the USP5 gene. USP5 is part of the ubiquitin specific protease family that cleaves ubiquitin from polyubiquitin chains. Its primary function is to disassemble unanchored polyubiquitin, recycling ubiquitin monomers for reuse in the ubiquitin proteasome system. Through this activity, USP5 maintains ubiquitin homeostasis, regulates protein turnover, and influences signaling pathways that depend on ubiquitination.\u003cbr\u003e\u003cbr\u003eUSP5 antibody is commonly used in research on protein degradation, signal transduction, and cancer biology. By detecting USP5, scientists can study how ubiquitin processing enzymes control proteostasis and prevent accumulation of abnormal proteins. Dysregulation of USP5 activity leads to disruptions in protein quality control and contributes to disease.\u003cbr\u003e\u003cbr\u003eIn western blot assays, USP5 antibody detects protein bands corresponding to the enzyme in cell and tissue extracts. Immunohistochemistry reveals localization in cytoplasmic and nuclear compartments, while immunofluorescence highlights its presence in proteasome rich regions. These applications make USP5 antibody versatile for tracking ubiquitin protease activity.\u003cbr\u003e\u003cbr\u003eUSP5 has been implicated in cancer progression, where it regulates stability of tumor suppressors and oncogenes. Elevated USP5 expression supports tumor cell survival and proliferation by maintaining ubiquitin pools and stabilizing growth promoting proteins. By applying USP5 antibody, researchers can assess how deubiquitinating enzymes contribute to malignancy and therapy resistance.\u003cbr\u003e\u003cbr\u003eBeyond cancer, USP5 plays roles in neurodegeneration, immune regulation, and viral infection. In neurons, altered USP5 activity contributes to proteotoxic stress and synaptic dysfunction. In immunity, it regulates signaling pathways dependent on ubiquitination, affecting cytokine responses and pathogen defense. This broad functional spectrum highlights the research value of USP5 antibody in multiple biological contexts.\u003cbr\u003e\u003cbr\u003eUSP5 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=USP5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=USP5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=USP5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/USP5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=USP5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047291380077,"sku":"FY12506","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_00a5982a-10f5-4cde-a5b3-a9d6d9fd6583.jpg?v=1772019301","url":"https:\/\/www.ebiohippo.com\/products\/usp5-antibody-ubiquitin-specific-peptidase-5-bha17135409","provider":"BioHippo","version":"1.0","type":"link"}