{"product_id":"vapa-antibody-vesicle-associated-membrane-protein-associated-protein-a-bha17135903","title":"VAPA Antibody \/ Vesicle-associated membrane protein-associated protein A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eVAPA Antibody \/ Vesicle-associated membrane protein-associated protein A is a anti-VAPA Rabbit antibody Recombinant Rabbit Monoclonal clone 32V29 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e VAPA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32V29, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eVAPA antibody detects Vesicle-associated membrane protein-associated protein A, encoded by the VAPA gene. Vesicle-associated membrane protein-associated protein A is an integral membrane protein localized mainly to the endoplasmic reticulum and involved in membrane trafficking, lipid transfer, and inter-organelle communication. It is part of the VAP protein family, which also includes VAPB, and plays a role in tethering endoplasmic reticulum membranes to other organelles, including the Golgi, mitochondria, and plasma membrane. VAPA antibody provides researchers with a critical tool to study intracellular transport, organelle homeostasis, and lipid signaling.\u003cbr\u003e\u003cbr\u003eVesicle-associated membrane protein-associated protein A interacts with multiple partners through its major sperm protein domain, including proteins containing FFAT motifs that mediate lipid exchange at membrane contact sites. Research using VAPA antibody has demonstrated that these contacts allow the direct transfer of lipids such as phosphoinositides, ceramides, and cholesterol between membranes, bypassing vesicular trafficking. This function is essential for maintaining lipid composition, signaling, and organelle identity. Dysregulation of these processes contributes to neurodegeneration, metabolic syndromes, and viral infection.\u003cbr\u003e\u003cbr\u003eIn virology, VAPA has been implicated in the life cycles of several viruses, including hepatitis C virus and enteroviruses, which hijack VAPA-dependent contact sites to establish replication organelles. Studies with VAPA antibody have shown that viral proteins interact with Vesicle-associated membrane protein-associated protein A to remodel endoplasmic reticulum membranes, creating environments conducive to viral replication. These findings highlight the relevance of VAPA in host-pathogen interactions and antiviral drug development.\u003cbr\u003e\u003cbr\u003eBeyond infectious disease, VAPA is also significant in neurodegeneration and metabolic regulation. Mutations or dysfunction in VAP proteins are linked to motor neuron diseases such as amyotrophic lateral sclerosis, and research using VAPA antibody has explored whether VAPA contributes to similar pathogenic mechanisms. In lipid metabolism, altered expression of VAPA affects cholesterol and sphingolipid distribution, linking it to metabolic disease and cardiovascular risk. These wide-ranging roles underscore the importance of studying VAPA in both normal physiology and pathology.\u003cbr\u003e\u003cbr\u003eVAPA antibody is widely used in western blotting, immunofluorescence, and immunoprecipitation. Western blotting reveals its expression in tissues such as brain, liver, and muscle. Immunofluorescence confirms its localization to endoplasmic reticulum membranes, often colocalizing with markers such as calnexin. Immunoprecipitation with VAPA antibody enables identification of interacting proteins, revealing new partners in lipid transfer and signaling pathways. These applications make it a versatile reagent across molecular and cellular studies.\u003cbr\u003e\u003cbr\u003eBy supplying validated VAPA antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=VAPA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=VAPA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=VAPA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/VAPA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=VAPA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309042029,"sku":"FY13001","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6584663d-39ce-49f6-b410-3041b5c6feb2.jpg?v=1772019377","url":"https:\/\/www.ebiohippo.com\/products\/vapa-antibody-vesicle-associated-membrane-protein-associated-protein-a-bha17135903","provider":"BioHippo","version":"1.0","type":"link"}