{"product_id":"vb6-vitamin-b6-competitive-elisa-kit-stje0006572-bhe15506641","title":"VB6 (Vitamin B6) Competitive ELISA Kit (STJE0006572)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eVB6 (STJE0006572)\u003c\/strong\u003e is a biomarker protein expressed in mammalian tissues and biological fluids. Dysregulation of STJE0006572 has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.\u003c\/p\u003e\n\u003cp\u003eQuantitative measurement of STJE0006572 enables researchers to characterize its abundance in Serum, Plasma and correlate levels with experimental conditions. This kit recognizes VB6 in samples. No significant cross-reactivity or interference between VB6 and analogues was observed.\u003c\/p\u003e\n\u003cp\u003eThis competitive ELISA kit provides a validated, reproducible format for STJE0006572 quantification within the inhibition range of 1.56~100ng\/mL. The assay supports experimental workflows in Cell Biology research.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eEach kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):\u003c\/p\u003e\n\u003cp\u003ePre-coated microplate (96 wells), HRP-conjugated antigen, standard protein, sample diluent, wash buffer, TMB substrate solution, stop solution, and product manual.\u003c\/p\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003eValidated Lot\u003c\/span\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eCompetitive ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56~100ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity (LLOD)\u003c\/th\u003e\n\u003ctd\u003e0.94ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidated Sample Types\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e2.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (450 nm \/ 540–570 nm reference)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e96-well microplate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eAll\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure Summary\u003c\/h2\u003e\n\u003cp\u003eAdd samples and HRP-conjugated antigen simultaneously to pre-coated wells and incubate. Wash, add TMB substrate, and read at 450 nm. Signal is inversely proportional to VB6 concentration. Construct a standard inhibition curve from the provided reference antigen to calculate sample concentrations. Total protocol time: approximately 2.5 hours.\u003c\/p\u003e\n\u003cp\u003ePre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eSt John's Laboratory Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003eSTJE0006572\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003eSee title\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"24 T","offer_id":53335887970669,"sku":"STJE0006572-24","price":203.55,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53335888757101,"sku":"STJE0006572-96","price":460.23,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":53335888789869,"sku":"STJE0006572-48","price":385.71,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/STJE0006572_1__42843.1631212876.jpg?v=1778912833","url":"https:\/\/www.ebiohippo.com\/products\/vb6-vitamin-b6-competitive-elisa-kit-stje0006572-bhe15506641","provider":"BioHippo","version":"1.0","type":"link"}