{"product_id":"viral-dna-rna-extraction-kit-bht15200019","title":"Viral DNA\/RNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eNucleic Acid Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe kit is suitable for simple, rapid and efficient separation and purification of DNA\/RNA from whole blood, tissue homogenate, swabs, serum, plasma and other acellular body fluids. The unique buffer system enables the viral nucleic acids in the lysate to bind to the silica gel centrifugal adsorbent column in a highly efficient and specific manner, so that the viral nucleic acids obtained are of high purity and stable quality, free of protein, nuclease and other impurities, and can be used in a variety of routine operations, including PCR, fluorescence quantitative PCR and other experiments.\u003c\/p\u003e\n\u003cp\u003eEach preparation combines 500 µL of Buffer RLC, 200 µL of sample and 20 µL of Proteinase K, vortexed for 5 s and mixed at 1200 rpm for 10 min. The lysate is loaded onto the Spin Column DM at 12,000 rpm for 1 min, washed with 500 µL of Buffer PGWT and 500 µL of Buffer GWT2, dried at 12,000 rpm for 2 min and air-dried for 2 min, then eluted with 40–100 µL of RNase-Free Water. The expected purity is OD260\/OD280 1.8–2.1, with 2.0 indicating high-quality RNA.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica gel centrifugal adsorbent column (Spin Column DM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eWhole blood, tissue homogenate, swabs, serum, plasma, other acellular body fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNucleic acids recovered\u003c\/th\u003e\n\u003ctd\u003eViral DNA and RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003e1.8–2.1; 2.0 indicates high-quality RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e40–100 µL RNase-Free Water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RLC\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PGWT\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GWT2\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Water\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpin Columns DM with Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Centrifuge Tubes (1.5 mL)\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eViral DNA and RNA purification from whole blood, serum and plasma\u003c\/li\u003e\n\u003cli\u003eNucleic acid purification from swabs and acellular body fluids\u003c\/li\u003e\n\u003cli\u003eNucleic acid purification from tissue homogenate\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for PCR and fluorescence quantitative PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eMost components are stored at room temperature for 1 year; Proteinase K is stored at -20°C for 1 year. Extracted nucleic acids are stored at -80°C for the long term.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBuffer RLC may crystallize; dissolve it at 56°C before use if needed.\u003c\/li\u003e\n\u003cli\u003eSwab samples require specific pre-treatment before lysis.\u003c\/li\u003e\n\u003cli\u003eAir-dry the column for 2 min after the drying spin before elution.\u003c\/li\u003e\n\u003cli\u003eStore extracted nucleic acids at -80°C for the long term.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP023\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200019\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512737133,"sku":"EP023-50T","price":312.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965515719021,"sku":"EP023-50TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP023-a-1.jpg?v=1789419115","url":"https:\/\/www.ebiohippo.com\/products\/viral-dna-rna-extraction-kit-bht15200019","provider":"BioHippo","version":"1.0","type":"link"}