{"product_id":"viral-rnaclean-kit-bht15200018","title":"Viral RNAclean Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eNucleic Acid Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis product is suitable for the extraction of various viral RNA or DNA from plasma, whole blood, cell-free body fluids (including plasma, serum, urine, CSF and cell culture supernatant), viral stock solution and infected tissues. After the nucleic acid released from the lysed virus binds to the purification column, the column is washed with Buffer WBR to remove residual PCR inhibitors, and the nucleic acid is then eluted with Buffer TE for PCR or RT-PCR.\u003c\/p\u003e\n\u003cp\u003eA 200 µL sample is combined with 20 µL of Protease K storage solution and 200 µL of Buffer REL containing Carrier RNA, vortexed for 15 s and incubated at 56°C for 10 min. After adding 320 µL of ethanol the mixture is loaded onto the nucleic acid purification column at 12,000 rpm for 30 s, washed with 700 µL of Buffer WBR, dried at 14,000 rpm for 1 min and eluted with 50 µL of preheated Buffer TE at 56°C. The extracted nucleic acid is stored at -20°C.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (nucleic acid purification column)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003ePlasma, whole blood, cell-free body fluids, viral stock solution, infected tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNucleic acids recovered\u003c\/th\u003e\n\u003ctd\u003eViral RNA or DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003eNo organic extraction or ethanol precipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eCarrier RNA supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e50 µL Buffer TE at 56°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNucleic acid purification column\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtease K storage solution\u003c\/td\u003e\n\u003ctd\u003e1.1 mL\u003c\/td\u003e\n\u003ctd\u003e4.4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCarrier RNA\u003c\/td\u003e\n\u003ctd\u003e400 µL\u003c\/td\u003e\n\u003ctd\u003e1.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer REL\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WBR\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TE\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eViral RNA and DNA extraction from plasma, serum and whole blood\u003c\/li\u003e\n\u003cli\u003eNucleic acid extraction from urine, CSF and cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eNucleic acid extraction from viral stock solution and infected tissue\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for PCR and RT-PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdd Carrier RNA for rapid purification to obtain high-quality viral RNA.\u003c\/li\u003e\n\u003cli\u003eNo organic extraction or ethanol precipitation.\u003c\/li\u003e\n\u003cli\u003eHigh repeatability and high output.\u003c\/li\u003e\n\u003cli\u003eRemoval of pollutants and inhibitors to facilitate downstream applications.\u003c\/li\u003e\n\u003cli\u003eThe manufacturer states that detection sensitivity can be increased by 10–50 times compared with the traditional boiling method.\u003c\/li\u003e\n\u003cli\u003eThe manufacturer states that detection sensitivity can be increased by 5–10 times compared with the traditional Trizol method.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eProtease K storage solution and Carrier RNA are stored at -20°C. Other reagents and materials keep their performance unchanged for two years at room temperature (15–25°C); storage at 2–8°C extends the product life beyond two years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse DNase-free and RNase-free tubes and filter pipette tips.\u003c\/li\u003e\n\u003cli\u003eAvoid contact between the solution and the edge of the column nozzle.\u003c\/li\u003e\n\u003cli\u003eConfirm that ethanol has been added to Buffer WBR before use.\u003c\/li\u003e\n\u003cli\u003eDo not omit the high-speed spin that removes residual ethanol.\u003c\/li\u003e\n\u003cli\u003eStore the extracted nucleic acid at -20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP017\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200018\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512212845,"sku":"EP017-50T","price":208.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965515424109,"sku":"EP017-200T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965515456877,"sku":"EP017-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965515489645,"sku":"EP017-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP017-a-1.jpg?v=1789419182","url":"https:\/\/www.ebiohippo.com\/products\/viral-rnaclean-kit-bht15200018","provider":"BioHippo","version":"1.0","type":"link"}