{"product_id":"vista-nfat-reporter-lentivirus-bhv19400279","title":"VISTA\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eVISTA (V-domain Ig suppressor of T cell activation) is an immune checkpoint receptor and ligand of the immunoglobulin superfamily, expressed predominantly on myeloid cells and, to a lesser extent, on T cells. VISTA delivers inhibitory signals that suppress T cell activation and proliferation and help maintain peripheral tolerance and a suppressive myeloid compartment. In tumors, VISTA contributes to an immunosuppressive microenvironment and immune evasion, making it an attractive checkpoint target. Because VISTA-mediated inhibition dampens NFAT-dependent transcription downstream of the T cell receptor, NFAT-driven reporters provide a quantitative readout of VISTA checkpoint signaling for immuno-oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe VISTA\/NFAT Reporter Lentivirus is an immunotherapy reporter system for studying VISTA checkpoint signaling. A receptor lentivirus constitutively expresses human VISTA with antibiotic selection, while a reporter lentivirus carries tandem NFAT response elements driving a dual reporter, secreted Gaussia luciferase and a fluorescent protein. Sequential transduction and selection generates a dual-stable cell line whose NFAT-driven reporter output reflects VISTA-modulated signaling.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase accumulates in conditioned media, enabling kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures. The system supports investigation of immune evasion, checkpoint inhibitor testing, and the role of VISTA in myeloid cell suppression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human VISTA under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251625025901,"sku":"TRV-0031-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_68aa1e02-dfc2-4cb1-ac66-0c8e697d20fb.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/vista-nfat-reporter-lentivirus-bhv19400279","provider":"BioHippo","version":"1.0","type":"link"}