{"product_id":"vu591-bhb21300367","title":"VU591","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 6-nitro-2-[(6-nitro-1H-benzimidazol-2-yl)methoxymethyl]-1H-benzimidazole.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 1222810-74-3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C16H12N6O5.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e ≥99%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 0.1-50 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized Powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e 50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Kir1.1 channel\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e VU591 is a Kir1.1 channel blocker1.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eVU591 is a potent and selective blocker of renal outer medullary potassium channel, ROMK (Kir1.1). Kir1.1 channel is a putative drug target for a novel class of loop diuretic drugs that hold the ability to lower pressure and blood volume without causing hypokalemia. VU591 seems to block the intracellular pore of the channel and does so with an IC50 of 300 nM.ROMK channels are expressed in the thick ascending limb of Henle's loop, connecting tubule, and collecting duct segments of the nephron. They mediate K+ secretion into the urinary filtrate. ROMK provides K+ ions in the thick ascending limb that are necessary for NaCl reabsorption by the Na+-K+-2Cl− cotransporter and loop diuretic target. In the connecting tubule and collecting duct, ROMK constitutes a key pathway for K+ secretion.Evidence shows that ROMK antagonists could serve as potent diuretic drugs by inhibiting Na+ reabsorption in the thick ascending limb of Henle's loop and minimizing urinary K+ ions loss in the collecting duct. Thus, administration of VU591, a direct diuretic, can avoid hypokalemia, a side effect caused from treatment of conventional loop and thiazide diuretics in cases of hypertension and congestive heart failure.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249715110253,"sku":"V-125-10MG-1","price":134.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311580111213,"sku":"V-125-25MG-1","price":297.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311580143981,"sku":"V-125-5MG-1","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/V-125_str-1.gif?v=1778544459","url":"https:\/\/www.ebiohippo.com\/products\/vu591-bhb21300367","provider":"BioHippo","version":"1.0","type":"link"}