{"product_id":"whole-blood-dna-miniprep-kit-bht15200009","title":"Whole Blood DNA Miniprep Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis product can rapidly separate and purify genomic DNA from 200–400 µL of fresh or frozen human or animal whole blood within 15–20 min. The product does not use proteinase K. After the whole blood is dissolved by Solution BFL1, the hemoglobin is removed by Solution BFL2 precipitation. The genomic DNA in the supernatant can be bound to the purification column. After being washed by Buffer RP and Wash Buffer, the remaining proteins on the membrane and the PCR inhibitors are removed, the genomic DNA is eluted by Elution Buffer and can be used in various molecular biology experiments immediately.\u003c\/p\u003e\n\u003cp\u003eIn the protocol, 300 µL of Solution BFL1 is added to 400 µL of blood and vortexed for 30 s, followed by 300 µL of Solution BFL2 and a further 30 s vortex. After centrifugation at 12,000 rpm for 2 min the supernatant is loaded onto Adsorption column G, washed with 500 µL of Buffer RP and twice with 600 µL of Wash Buffer, and eluted with 50–100 µL of Elution Buffer after 2–5 min. Purified DNA has an OD260\/OD280 of 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica gel membrane spin column (Adsorption column G)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eFresh or frozen human or animal whole blood, anticoagulated blood, buffy coat, poultry blood\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e200–400 µL whole blood\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e15–20 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003ePhenol\/chloroform-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProteinase K\u003c\/th\u003e\n\u003ctd\u003eNot used\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eOD260\/OD280 should be 1.7–1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e50–100 µL Elution Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution BFL1\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003ctd\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution BFL2\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003ctd\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RP\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003ctd\u003e120 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column G\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003ctd\u003e200 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenomic DNA extraction from whole blood\u003c\/li\u003e\n\u003cli\u003eDNA extraction from buffy coat and poultry blood\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFast and low toxicity: silica gel membrane adsorption, without phenol or chloroform.\u003c\/li\u003e\n\u003cli\u003eWide application: DNA can be directly extracted from samples such as anticoagulant blood, buffy coat and poultry blood.\u003c\/li\u003e\n\u003cli\u003eHigh quality: The purified DNA has the characteristics of high concentration, high purity, and good integrity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at room temperature for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles of blood samples.\u003c\/li\u003e\n\u003cli\u003eDo not use less than 400 µL of anticoagulated whole blood per preparation.\u003c\/li\u003e\n\u003cli\u003eVortex for 30 s after adding Solution BFL1 and again after adding Solution BFL2.\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the Wash Buffer before use.\u003c\/li\u003e\n\u003cli\u003eCentrifuge the column for 2 min to dry it before elution.\u003c\/li\u003e\n\u003cli\u003eA second elution can be carried out to increase yield.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP008\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200009\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"200 T","offer_id":53965511852397,"sku":"EP008-200T","price":260.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965514867053,"sku":"EP008-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965514899821,"sku":"EP008-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP008-a-1.jpg?v=1789419311","url":"https:\/\/www.ebiohippo.com\/products\/whole-blood-dna-miniprep-kit-bht15200009","provider":"BioHippo","version":"1.0","type":"link"}