{"product_id":"xpo1-antibody-exportin-1-crm1-bha17109325","title":"XPO1 Antibody \/ Exportin 1 \/ CRM1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eXPO1 Antibody \/ Exportin 1 \/ CRM1 is a research-use antibody directed against \u003cstrong\u003eXPO1\u003c\/strong\u003e. It is supplied for use in common immunoassay contexts such as IHC-P, WB, FACS, IF (RUO).\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e XPO1.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eDescription (provided):\u003c\/strong\u003e Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eAntibody type:\u003c\/strong\u003e Mouse, clone 5G3, Mouse IgG2b.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified; Protein G affinity.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eReported\/predicted localization:\u003c\/strong\u003e Nucleus, Cytoplasm.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e tested: Human, Mouse, Rat.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eImmunogen (if provided):\u003c\/strong\u003e Human Exportin 1 recombinant protein (amino acids N966-D1071) was used as the immunogen for the XPO1 antibody..\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe information above helps you match the antibody format to your assay context, interpret species-dependent differences, and anticipate how epitope context (isoforms, PTMs, or conformational state) may influence signal.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eExportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.\u003c\/p\u003e\n\u003cp\u003eFor curated annotations (gene\/protein naming, domains, isoforms, and pathway links) for XPO1, consult primary databases such as UniProt, NCBI Gene, and Ensembl.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \n\u003cli\u003eContext-dependent expression studies: researchers often examine XPO1 abundance and localization across perturbations (genetic, pharmacologic, or environmental) to connect phenotype to molecular changes.\u003c\/li\u003e  \u003cli\u003eReagent reproducibility: there is growing emphasis on antibody specificity checks using orthogonal approaches (e.g., genetic perturbation or independent antibodies) and transparent reporting of clone\/lot information.\u003c\/li\u003e  \u003cli\u003eMulti-modal datasets: antibody-based readouts are increasingly combined with transcriptomics and imaging to relate protein-level measurements to cell-state transitions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \n\u003cli\u003eImmunohistochemistry for spatial mapping of target expression across tissues and cell types.\u003c\/li\u003e  \u003cli\u003eWestern blotting (immunoblot) for relative detection of target protein abundance and apparent molecular weight.\u003c\/li\u003e  \u003cli\u003eFACS: commonly used to detect or compare XPO1 across experimental conditions (conceptual guidance only).\u003c\/li\u003e  \u003cli\u003eImmunofluorescence for subcellular localization and cell-type specific expression patterns.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen comparing conditions, interpret changes in signal in the context of sample composition, expected localization, and any known isoform complexity for the target.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \n\u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e alternative splicing or post-translational modifications can change epitope accessibility and apparent molecular weight; interpret bands\/signals accordingly.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and matrix effects:\u003c\/strong\u003e background binding can vary by sample type, species, and blocking\/detection chemistries; include appropriate negative controls.\u003c\/li\u003e  \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e where feasible, use genetic perturbation (KO\/KD\/overexpression), orthogonal assays, or independent antibodies to support specificity claims.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAntibody considerations:\u003c\/strong\u003e Polyclonal reagents may recognize multiple epitopes and can increase sensitivity but may show broader binding profiles, while monoclonal clones provide a single-epitope readout that can improve consistency across experiments. If a conjugate is listed, the antibody supports more direct detection workflows; otherwise, it is typically used with a compatible secondary antibody.\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProtKB entry for XPO1 (UniProt): https:\/\/www.uniprot.org\/uniprotkb\/O14980\n- NCBI Gene search for XPO1 (NCBI): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=XPO1\n- Ensembl gene search for XPO1 (Ensembl): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=XPO1\n- Antibody validation “5 pillars” (Nature Methods, 2016): https:\/\/www.nature.com\/articles\/nmeth.3995\n- NIH replication \u0026 reproducibility resources (NIH): https:\/\/www.nih.gov\/replicationandreproducibility\n- Human Protein Atlas search for XPO1 (HPA): https:\/\/www.proteinatlas.org\/search\/XPO1\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53044470350189,"sku":"RQ4517","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_97b5ac4c-7504-4166-b4c9-dff4e0e36df8.jpg?v=1771938916","url":"https:\/\/www.ebiohippo.com\/products\/xpo1-antibody-exportin-1-crm1-bha17109325","provider":"BioHippo","version":"1.0","type":"link"}