{"product_id":"zap-70-pe-bha19900836","title":"ZAP-70 PE","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZAP-70 PE is a research monoclonal targeting ZAP-70, supplied as a PE format for FC workflows. It supports measurement of Human target expression in common experimental systems.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClone:\u003c\/strong\u003e C-D12 — consistent clone identity can support panel reproducibility and cross-study comparisons.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG1, k — informs selection of matched controls and secondary reagents when relevant.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e PE — enables direct detection in fluorescence-based assays. Excitation is typically matched to Blue (488nm) lasers in cytometer configurations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human — interpret staining in the context of species-specific sequence and expression differences.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eKey specifications such as clone identity, isotype, and fluorophore conjugation help researchers align panel design, control selection, and instrument configuration with the biological question and sample type.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe clone C-D12, a mouse monoclonal antibody selectively binds with the 70kd tyrosine phosphoprotein known as ZAP-70 which is usually associated with the ζ -chain CD3-receptor-associated protein tyrosine kinase (PTK). It is a member of the Syk family that is localized exclusively to the cytosol of T cells and natural killer (NK) cells and is required for their cellular activation. It is generally considered the T-lymphocyte counterpart of Syk, a B-cell receptor (BCR)-associated kinase that belongs to the same PTK family and plays a similar role in the antigen receptor signaling in the B-lineage cells. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. ZAP-70 expression plays an important role in the clinical classification of various types of hematologic malignancies.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eHigh-parameter immunophenotyping: combining ZAP-70 with complementary lineage and activation markers to resolve complex cell states.\u003c\/li\u003e\n\u003cli\u003ePanel standardization and data comparability: increasing emphasis on consistent reagents, compensation-aware fluorophore choices, and shared gating strategies.\u003c\/li\u003e\n\u003cli\u003eIntegration with single-cell multi-omics: pairing surface marker profiling with transcriptomic or proteomic readouts to connect phenotype to function.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eFlow cytometry: quantify ZAP-70-positive populations and compare expression distributions across conditions or time points.\u003c\/li\u003e\n\u003cli\u003eCell sorting: enrich ZAP-70-defined subsets for downstream RNA\/protein assays or functional readouts.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eChanges in measured signal are typically interpreted in the context of cell subset frequency, activation\/differentiation state, and sample processing effects rather than as a standalone readout.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eFluorophore selection: consider brightness, spectral overlap, and instrument configuration; compensation and spillover can affect apparent population boundaries.\u003c\/li\u003e\n\u003cli\u003eBiology-driven confounders: activation state, differentiation, and isoform\/PTM variation can shift epitope accessibility and apparent expression.\u003c\/li\u003e\n\u003cli\u003eControl concepts: include matched isotype and fluorescence-minus-one (FMO) controls where appropriate, and interpret results alongside biological positive\/negative reference samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFor antibody-based assays, monoclonal versus polyclonal format can influence epitope recognition breadth and signal consistency. Conjugated antibodies support direct detection and can simplify multicolor panel design when paired with appropriate controls and instrument settings.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase — UniProt — https:\/\/www.uniprot.org\/ - NCBI Gene — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - HGNC gene nomenclature — HUGO Gene Nomenclature Committee — https:\/\/www.genenames.org\/ - Flow cytometry basics — NIH\/NCI (overview resources) — https:\/\/www.cancer.gov\/research\/resources - High-dimensional cytometry overview — Nature Methods (journal) — https:\/\/www.nature.com\/nmeth\/ --\u003e","brand":"Caprico","offers":[{"title":"25 Tests","offer_id":53072779051373,"sku":"120324","price":105.0,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":53072854745453,"sku":"120325","price":230.0,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":53072854778221,"sku":"120326","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20210216-ZAP70-C-D12-PE-203SE1-FV.jpg?v=1772634916","url":"https:\/\/www.ebiohippo.com\/products\/zap-70-pe-bha19900836","provider":"BioHippo","version":"1.0","type":"link"}