{"product_id":"zc3h12a-antibody-bha10809571","title":"ZC3H12A antibody","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePolyclonal Antibody\u003c\/span\u003e\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eEndoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay(PubMed:19909337). Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation(PubMed:26320658). Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines(IL6 and IL2), cell surface receptors(ICOS, TNFRSF4 and TNFR2) and transcription factor(REL)(By similarity). Self regulates by destabilizing its own mRNA(By similarity). Cleaves mRNA harboring a stem-loop(SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner(PubMed:19909337, PubMed:26320658, PubMed:26134560, PubMed:22561375). Plays a role in the inhibition of microRNAs(miRNAs) biogenesis(PubMed:22055188). Cleaves the terminal loop of a set of precursor miRNAs(pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs(PubMed:22055188). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity(PubMed:24048733). Affects the overall ubiquitination of cellular proteins(By similarity). Positively regulates deubiquitinase activity promoting the cleavage at 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors(TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulating macrophage-mediated inflammatory response and immune homeostasis(By similarity). Induces also deubiquitination of the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes(PubMed:24048733). Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989). Prevents stress granule(SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock and energy deprivation(By similarity). Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state(By similarity). May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis(PubMed:16574901, PubMed:18364357). Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein(APP)-dependent signaling pathway(PubMed:19185603). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide(LPS) by reducing I-kappa-B-kinase(IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production(By similarity). \n(Microbial infection) Exhibits broad antiviral activity by cleaving viral RNAs(PubMed:23355615). Binds to Japanese encephalitis virus(JEV) and dengue virus(DEN) RNAs(PubMed:23355615). Exhibits antiviral activity against HIV-1 in lymphocytes by decreasing the abundance of HIV-1 viral RNA species(PubMed:24191027).\u003c\/p\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis polyclonal antibody, raised in Rabbit, recognizes ZC3H12A. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Western Blot, Immunohistochemistry, Immunoprecipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eWB\u003c\/th\u003e\n\u003ctd\u003e1:200-1:1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIHC\u003c\/th\u003e\n\u003ctd\u003e1:20-1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIP\u003c\/th\u003e\n\u003ctd\u003e1:200-1:1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eOptimal dilutions should be determined by the end user for each application and sample type.\u003c\/p\u003e\n\u003ch2\u003eImmunogen\u003c\/h2\u003e\n\u003cp\u003ezinc finger CCCH-type containing 12A\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen\u003c\/th\u003e\n\u003ctd\u003ezinc finger CCCH-type containing 12A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eImmunogen Affinity Purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e≥95% as determined by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget MW\u003c\/th\u003e\n\u003ctd\u003e66 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt #\u003c\/th\u003e\n\u003ctd\u003eQ5D1E8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene ID\u003c\/th\u003e\n\u003ctd\u003e80149\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze \/ thaw cycles.)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eEndoribonuclease ZC3H12A|Monocyte chemotactic protein-induced protein 1 (MCP-induced protein 1, MCPIP-1)|Regnase-1 (Reg1)|Zinc finger CCCH domain-containing protein 12A|ZC3H12A|MCPIP|MCPIP1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eValidated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eThis product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore related antibodies and reagents for ZC3H12A research from Fine Test, or contact us for alternative formats.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Fine Test","offers":[{"title":"100 ug","offer_id":53750127395181,"sku":"FNab09601-100UG","price":364.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FNab09601.jpg?v=1786991386","url":"https:\/\/www.ebiohippo.com\/products\/zc3h12a-antibody-bha10809571","provider":"BioHippo","version":"1.0","type":"link"}