{"product_id":"zebrafish-alcama-antibody-neurolin-bha17136396","title":"Zebrafish Alcama Antibody \/ Neurolin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Alcama Antibody \/ Neurolin is a anti-Zebrafish Alcama Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Alcama\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eActivated leukocyte cell adhesion molecule a (Alcama), also known as Neurolin, is a member of the immunoglobulin superfamily of cell adhesion molecules. In zebrafish (Danio rerio), Alcama plays a vital role in axon guidance, cell-cell adhesion, and tissue morphogenesis, particularly during the development of the nervous system, vasculature, and hematopoietic system.\u003cp\u003eAlcama is prominently expressed in developing neurons, where it mediates axon fasciculation and growth cone navigation, facilitating the formation of organized neuronal tracts. In addition to its role in neurodevelopment, zebrafish Alcama is expressed in vascular endothelial cells and hematopoietic stem and progenitor cells (HSPCs), where it contributes to vasculogenesis, vascular remodeling, and stem cell niche formation.\u003c\/p\u003e\n\u003cp\u003eDue to its conserved structure and multifunctional roles, zebrafish Alcama is a valuable model for studying cell adhesion dynamics, nervous system wiring, hematopoietic development, and angiogenesis. It is also used in research related to immune cell migration, axon pathfinding disorders, and regenerative medicine.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Alcama - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Alcama - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Alcama - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Alcama - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Alcama+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047328047469,"sku":"RZ1201","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_78bea3b5-01cc-484e-b972-f80d3b45ab70.jpg?v=1772019457","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-alcama-antibody-neurolin-bha17136396","provider":"BioHippo","version":"1.0","type":"link"}