{"product_id":"zebrafish-caspase-9-antibody-casp9-bha17136403","title":"Zebrafish Caspase 9 Antibody \/ Casp9","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Caspase 9 Antibody \/ Casp9 is a anti-CASP9 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CASP9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCaspase 9 is a key initiator caspase in the intrinsic (mitochondrial) apoptotic pathway, responsible for activating downstream effector caspases and orchestrating programmed cell death. In zebrafish (Danio rerio), Caspase 9 plays a conserved role in regulating apoptosis during embryonic development, as well as in maintaining tissue homeostasis and responding to cellular stress.\u003cp\u003eFollowing mitochondrial outer membrane permeabilization, cytochrome c is released into the cytosol, leading to the formation of the apoptosome complex. Caspase 9 is recruited and activated within this complex, initiating a proteolytic cascade that culminates in the activation of effector caspases such as Caspase 3 and Caspase 7. In zebrafish, casp9 is expressed in multiple tissues during development and is crucial for eliminating damaged, mis-specified, or excess cells.\u003c\/p\u003e\n\u003cp\u003eZebrafish Caspase 9 is widely used in research on cell death mechanisms, neurodevelopment, cancer biology, and toxicology, providing an in vivo model to study mitochondria-mediated apoptosis and the effects of pro- and anti-apoptotic signals. Due to its high degree of functional conservation with mammalian caspase-9, it serves as a valuable tool for both basic and translational research.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CASP9 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CASP9 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CASP9 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CASP9 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CASP9+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327719789,"sku":"RZ1208","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e5ae165f-297d-4e8e-a11b-84143298e2b8.jpg?v=1772019456","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-caspase-9-antibody-casp9-bha17136403","provider":"BioHippo","version":"1.0","type":"link"}