{"product_id":"zebrafish-cdc48-antibody-valosin-containing-protein-vcp-bha17136517","title":"Zebrafish Cdc48 Antibody \/ Valosin-containing protein \/ Vcp","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Cdc48 Antibody \/ Valosin-containing protein \/ Vcp is a anti-CDC48 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CDC48\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Cdc48, also known as cell division cycle 48, is a crucial ATPase that is involved in various cellular processes including protein degradation, cell cycle regulation, and membrane dynamics. Cdc48 is part of the AAA+ ATPase family and functions as a part of the 26S proteasome pathway, where it helps to extract polyubiquitinated substrates from the proteasome or other cellular compartments for degradation. Additionally, Cdc48 plays a role in endoplasmic reticulum associated degradation (ERAD), maintaining cellular homeostasis by ensuring the removal of misfolded proteins.\u003cp\u003eZebrafish Cdc48 is an ortholog of the human CDC48 protein, also known as p97. It shares significant sequence and functional conservation with its human counterpart, ensuring that it performs similar roles in regulating protein turnover, signaling pathways, and cell cycle progression. Like human p97, zebrafish Cdc48 is essential for the regulation of processes such as DNA repair, organelle biogenesis, and apoptosis, and its dysfunction can result in various diseases, including neurodegenerative disorders and certain cancers.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Cdc48 antibody is an invaluable tool for studying protein quality control mechanisms and their role in cellular stress responses. Such antibodies are essential for applications including western blot, immunohistochemistry, immunofluorescence, and ELISA. With a Zebrafish Cdc48 antibody, researchers can analyze Cdc48 expression in different tissues, study its involvement in proteostasis, and investigate how alterations in its function impact development and disease.\u003c\/p\u003e\n\u003cp\u003eCurrently, no isoforms of zebrafish Cdc48 have been documented, but its essential role in cellular maintenance and its conservation across species make it an important target for research. A high-quality Zebrafish Cdc48 antibody provides essential insights into cellular stress mechanisms and the regulation of the ubiquitin-proteasome system in zebrafish models.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CDC48 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CDC48 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CDC48 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CDC48 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CDC48+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331717485,"sku":"RZ1323","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_636fcda4-59e6-444c-bffd-f7181d1dab96.jpg?v=1772019478","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-cdc48-antibody-valosin-containing-protein-vcp-bha17136517","provider":"BioHippo","version":"1.0","type":"link"}