{"product_id":"zebrafish-cdkn1a-antibody-p21-waf1-cip1-bha17136453","title":"Zebrafish Cdkn1a Antibody \/ p21 \/ Waf1 \/ Cip1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Cdkn1a Antibody \/ p21 \/ Waf1 \/ Cip1 is a anti-CDKN1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CDKN1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCdkn1a, also known as cyclin dependent kinase inhibitor 1a, is a key regulatory protein that controls cell cycle progression by inhibiting cyclin dependent kinases. It plays an essential role in maintaining the balance between cell proliferation and cell cycle arrest, particularly in response to DNA damage and other cellular stress signals.\u003cp\u003eIn zebrafish, Cdkn1a is expressed in various tissues during embryogenesis and in adult organs, especially in regions undergoing rapid cell division or experiencing genotoxic stress. Zebrafish Cdkn1a is a confirmed ortholog of the human CDKN1A gene, which encodes the protein commonly known as p21. Both zebrafish and human Cdkn1a proteins are structurally conserved and share similar mechanisms of action in regulating the cell cycle and promoting cellular senescence or apoptosis.\u003c\/p\u003e\n\u003cp\u003eCdkn1a is a downstream effector of the tumor suppressor protein p53 and becomes upregulated following DNA damage or oxidative stress. It functions primarily by binding to and inhibiting cyclin E and cyclin A associated kinases, thereby enforcing a cell cycle arrest at the G one and S phase transition. In zebrafish, Cdkn1a is widely used as a biomarker for DNA damage response and is essential for proper embryonic development, tissue regeneration, and maintenance of genomic stability.\u003c\/p\u003e\n\u003cp\u003eDue to its evolutionary conservation and central role in cell cycle control and stress response, zebrafish Cdkn1a is a valuable model for studying cancer biology, toxicology, aging, and regenerative medicine. It is often used in research investigating p53 signaling, chemical screening, and genetic regulation of cell cycle arrest.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CDKN1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CDKN1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CDKN1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CDKN1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CDKN1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330046317,"sku":"RZ1258","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_36d73be9-0aab-4ade-a65d-0e5196e3cb9a.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-cdkn1a-antibody-p21-waf1-cip1-bha17136453","provider":"BioHippo","version":"1.0","type":"link"}