{"product_id":"zebrafish-ckmb-creatine-kinase-mb-isoenzyme-elisa-kit-bhe15212978","title":"Zebrafish CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eCKMB (Creatine Kinase MB Isoenzyme)\u003c\/strong\u003e is associated with intracellular signaling regulation, often within phosphorylation-dependent pathways that control activation, proliferation, or differentiation.\u003c\/p\u003e\u003cp\u003eWhile kinase activity is frequently assessed by phospho-readouts, total protein abundance can also shift with pathway rewiring, feedback, or changes in cell composition.\u003c\/p\u003e\u003cp\u003eProtein quantification can complement phospho-specific assays and help interpret whether signaling changes are driven by abundance, activation state, or both.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eCKMB (Creatine Kinase MB Isoenzyme)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eZebrafish\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 1.57-100 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.57 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966472384877,"sku":"ELK11392-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966472417645,"sku":"ELK11392-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966472450413,"sku":"ELK11392-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_4dc8a0a7-79e1-4574-8aff-6fb256e75bfc.jpg?v=1769437106","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-ckmb-creatine-kinase-mb-isoenzyme-elisa-kit-bhe15212978","provider":"BioHippo","version":"1.0","type":"link"}