{"product_id":"zebrafish-dgcr8-antibody-bha17136412","title":"Zebrafish Dgcr8 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Dgcr8 Antibody is a anti-DGCR8 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Immunofluorescence (IF), IHC-P with listed reactivity in Zebrafish. Reported localization: Nucleus, Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DGCR8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IF, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDgcr8, or DiGeorge syndrome critical region gene eight, is a double stranded RNA binding protein that plays a key role in the microRNA processing pathway. In zebrafish, Dgcr8 is an essential component of the microprocessor complex, which includes the ribonuclease enzyme Drosha. Together, they initiate the first step in converting primary microRNA transcripts into precursor microRNAs, which are further processed into mature microRNAs.\u003cp\u003eMicroRNAs are short noncoding RNAs that regulate gene expression by targeting messenger RNAs for degradation or translational repression. Dgcr8 is crucial for maintaining proper levels of microRNAs, which are involved in numerous biological processes including early development, cell differentiation, neurogenesis, and organ formation.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Dgcr8 is expressed during early embryogenesis and is required for normal development of the brain, heart, and other organs. Loss of Dgcr8 function results in widespread defects due to disrupted microRNA biogenesis. Because of its evolutionary conservation and central role in RNA regulation, zebrafish Dgcr8 is widely used in studies of gene expression control, developmental biology, and disease models related to microRNA dysfunction.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DGCR8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DGCR8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DGCR8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DGCR8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DGCR8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329030509,"sku":"RZ1217","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e984ebd1-0d90-4a57-97ba-8fdf92c04744.jpg?v=1772019458","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-dgcr8-antibody-bha17136412","provider":"BioHippo","version":"1.0","type":"link"}