{"product_id":"zebrafish-gata4-antibody-gata-binding-factor-4-bha17136381","title":"Zebrafish Gata4 Antibody \/ GATA-binding factor 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Gata4 Antibody \/ GATA-binding factor 4 is a anti-GATA4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GATA4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGATA-binding factor 4 (Gata4) is a zinc finger transcription factor belonging to the GATA family, which regulates gene expression during embryonic development and cell differentiation. In zebrafish (Danio rerio), Gata4 plays a critical role in the development of the heart, endoderm, and gastrointestinal tract, mirroring its conserved function in vertebrate organogenesis.\u003cp\u003eGata4 is expressed early during zebrafish embryogenesis, particularly in the cardiac mesoderm and endodermal precursors. It functions as a key regulator of cardiomyocyte differentiation, heart tube formation, and gut development. Gata4 also interacts with other transcription factors such as Nkx2.5 and Tbx5 to coordinate cardiac gene expression programs.\u003c\/p\u003e\n\u003cp\u003eDue to its essential role in heart development and its evolutionary conservation, zebrafish Gata4 is widely studied in models of congenital heart disease, organ specification, and regenerative biology. It serves as a valuable molecular marker and research target for developmental biology, gene regulation, and disease modeling.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GATA4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GATA4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GATA4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GATA4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GATA4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327818093,"sku":"RZ1186","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f60e5f97-d4d5-4687-b8cb-8c8ab0e79878.jpg?v=1772019453","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-gata4-antibody-gata-binding-factor-4-bha17136381","provider":"BioHippo","version":"1.0","type":"link"}