{"product_id":"zebrafish-hbae1-1-antibody-hemoglobin-alpha-embryonic-1-1-bha17136423","title":"Zebrafish Hbae1.1 Antibody \/ Hemoglobin alpha embryonic 1.1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Hbae1.1 Antibody \/ Hemoglobin alpha embryonic 1.1 is a anti-Zebrafish Hbae1.1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Hbae1.1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHbae1.1, or hemoglobin alpha embryonic 1.1, is a subunit of embryonic hemoglobin expressed during the early stages of zebrafish development. Hemoglobins are iron binding proteins responsible for oxygen transport in the blood, and in zebrafish, different hemoglobin genes are activated in a stage specific manner to meet the oxygen needs of the developing embryo.\u003cp\u003eHbae1.1 is expressed primarily in the intermediate cell mass, which is the site of primitive erythropoiesis in zebrafish embryos. It forms part of the embryonic hemoglobin complex that enables efficient oxygen delivery during rapid cell growth and organ formation. As development progresses, Hbae1.1 expression decreases while adult forms of hemoglobin become dominant.\u003c\/p\u003e\n\u003cp\u003eDue to its specific and transient expression pattern, Hbae1.1 is widely used as a molecular marker for primitive erythroid cells and early blood development in zebrafish. It provides a valuable readout for studying hematopoiesis, gene regulation during red blood cell formation, and the effects of genetic mutations or chemical exposures on early blood cell differentiation.\u003c\/p\u003e\n\u003cp\u003eZebrafish Hbae1.1 is an important tool in developmental biology, hematology, toxicology, and genetic screening for blood related disorders.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Hbae1.1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Hbae1.1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Hbae1.1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Hbae1.1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Hbae1.1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047328506221,"sku":"RZ1228","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4f33fba4-2c4e-46ff-8194-2550a82a6b19.jpg?v=1772019461","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-hbae1-1-antibody-hemoglobin-alpha-embryonic-1-1-bha17136423","provider":"BioHippo","version":"1.0","type":"link"}