{"product_id":"zebrafish-hsd3b1-antibody-bha17136393","title":"Zebrafish Hsd3b1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Hsd3b1 Antibody is a anti-HSD3B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HSD3B1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003e3-beta-hydroxysteroid dehydrogenase type 1 (Hsd3b1) is a key enzyme in the biosynthesis of steroid hormones, catalyzing the conversion of delta-5-3-beta-hydroxysteroids to delta-4-ketosteroids. In zebrafish (Danio rerio), Hsd3b1 plays a crucial role in the production of glucocorticoids, mineralocorticoids, androgens, and estrogens, and is essential for proper steroidogenic tissue function, including that of the gonads and interrenal gland (the fish analog of the adrenal cortex).\u003cp\u003eZebrafish hsd3b1 is expressed in steroidogenic tissues during development and in adult stages, where it supports endocrine functions related to stress response, reproduction, and growth. The enzyme is highly conserved across vertebrates, making zebrafish a valuable model for studying steroid biosynthesis, hormonal regulation, and the effects of endocrine-disrupting chemicals (EDCs).\u003c\/p\u003e\n\u003cp\u003eBecause of its involvement in hormone production and its responsiveness to physiological and environmental signals, zebrafish Hsd3b1 is widely used in research on developmental endocrinology, reproductive biology, and toxicology.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HSD3B1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HSD3B1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HSD3B1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HSD3B1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HSD3B1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047328375149,"sku":"RZ1198","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e53aea83-6a42-4df6-a8b3-a491c7cb3da7.jpg?v=1772019455","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-hsd3b1-antibody-bha17136393","provider":"BioHippo","version":"1.0","type":"link"}