{"product_id":"zebrafish-hsp60-antibody-hspd1-bha17130591","title":"Zebrafish Hsp60 Antibody \/ Hspd1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Hsp60 Antibody \/ Hspd1 is an antibody targeting \u003cstrong\u003eHsp60\u003c\/strong\u003e. Host: \u003cstrong\u003eRabbit\u003c\/strong\u003e. Polyclonal. Format: Antigen affinity purified. Applications: WB, IHC-P, IF.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Hsp60.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e An E.coli-derived zebrafish HSP60\/HSPD1 recombinant protein (amino acids K72-Q496) was used as the immunogen for the Ze….\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Zebrafish.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasm.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated (no label listed).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background (from provided description)\u003c\/h2\u003e\u003cp\u003eHSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive\/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e relative abundance and band pattern assessment using appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e tissue distribution assessment; interpret with matched controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIF\/ICC:\u003c\/strong\u003e subcellular localization imaging with proper background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAccount for isoforms, paralogs, and post-translational modifications when interpreting signal.\u003c\/li\u003e\n\u003cli\u003eSupport specificity with biological controls (e.g., KO\/KD where available) and technical controls (e.g., secondary-only\/isotype).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt) – https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NLM) – https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - ZFIN – https:\/\/zfin.org\/ - Ensembl – https:\/\/www.ensembl.org\/ - Nature Methods (2015): A proposal for validation of antibodies – https:\/\/www.nature.com\/articles\/nmeth.3230 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53046640738669,"sku":"RZ1163","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_66e6025d-02b6-4eee-8120-0d477cf34f33.jpg?v=1772009921","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-hsp60-antibody-hspd1-bha17130591","provider":"BioHippo","version":"1.0","type":"link"}