{"product_id":"zebrafish-hspa5-antibody-grp78-bip-bha17136376","title":"Zebrafish Hspa5 Antibody \/ Grp78 \/ Bip","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Hspa5 Antibody \/ Grp78 \/ Bip is a anti-HSPA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HSPA5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHeat shock 70 kDa protein 5 (Hspa5), also known as GRP78 (Glucose-Regulated Protein 78) or BiP (Binding Immunoglobulin Protein), is a highly conserved member of the HSP70 family of molecular chaperones. In zebrafish (Danio rerio), hspa5 encodes an endoplasmic reticulum (ER)-resident chaperone that plays a central role in protein quality control, particularly during ER stress and the unfolded protein response (UPR).\u003cp\u003eHspa5 facilitates proper protein folding, prevents aggregation of misfolded proteins, and targets irreversibly misfolded proteins for degradation. It is also a key regulator of ER homeostasis, acting as a sensor for misfolded proteins and an upstream effector in activating UPR pathways, including PERK, ATF6, and IRE1.\u0026lt;\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, hspa5 is expressed ubiquitously during early embryogenesis and is upregulated in response to physiological and environmental stressors such as hypoxia, oxidative stress, and toxin exposure. Its expression is especially prominent in secretory tissues and rapidly developing organs such as the liver, pancreas, and brain.\u003c\/p\u003e\n\u003cp\u003eZebrafish Hspa5 serves as a useful marker for ER stress and has been widely used in models of developmental defects, neurodegeneration, metabolic disease, and toxicology screening. Due to its evolutionary conservation and functional parallels to mammalian GRP78, zebrafish Hspa5 is a valuable target for studying cellular stress responses and disease mechanisms in vivo.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HSPA5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HSPA5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HSPA5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HSPA5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HSPA5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327129965,"sku":"RZ1181","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9b8820cf-21c1-49e7-86c5-3a215c2526b0.jpg?v=1772019454","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-hspa5-antibody-grp78-bip-bha17136376","provider":"BioHippo","version":"1.0","type":"link"}