{"product_id":"zebrafish-hypoxanthine-phosphoribosyltransferase-antibody-hprt1-bha17130539","title":"Zebrafish Hypoxanthine phosphoribosyltransferase Antibody \/ Hprt1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eZebrafish Hypoxanthine phosphoribosyltransferase Antibody \/ Hprt1 is an antibody targeting \u003cstrong\u003eZebrafish Hypoxanthine phosphoribosyltransferase\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Hypoxanthine phosphoribosyltransferase.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); Rabbit Ig.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Zebrafish.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB, IHC-P (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eHypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eImmunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry Q7ZV49 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q7ZV49 - Gene search: Zebrafish Hypoxanthine phosphoribosyltransferase — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Hypoxanthine+phosphoribosyltransferase - Ensembl search: Zebrafish Hypoxanthine phosphoribosyltransferase — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Hypoxanthine+phosphoribosyltransferase - PubMed search: Zebrafish Hypoxanthine phosphoribosyltransferase antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Hypoxanthine+phosphoribosyltransferase+antibody - Reactome search: Zebrafish Hypoxanthine phosphoribosyltransferase — Reactome — https:\/\/reactome.org\/content\/query?q=Zebrafish+Hypoxanthine+phosphoribosyltransferase --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53046497575277,"sku":"RZ1111","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e5fa4cf4-18ca-45c8-abf4-b81067504f6c.jpg?v=1772000956","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-hypoxanthine-phosphoribosyltransferase-antibody-hprt1-bha17130539","provider":"BioHippo","version":"1.0","type":"link"}