{"product_id":"zebrafish-kita-antibody-kit-scfr-bha17136379","title":"Zebrafish Kita Antibody \/ Kit \/ Scfr","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Kita Antibody \/ Kit \/ Scfr is a anti-Zebrafish Kita Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Kita\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe Kit protein in zebrafish (Danio rerio), encoded by the kit gene (also referred to as kita or kitb depending on isoform), is a homolog of the mammalian c-Kit receptor, a type III receptor tyrosine kinase. Kit plays an essential role in multiple developmental processes, including melanocyte formation, germ cell development, and hematopoiesis.\u003cp\u003eZebrafish possess two paralogs of the kit gene due to a teleost-specific genome duplication: kita and kitb. Among them, kita is primarily responsible for the development of melanophores, the pigment cells responsible for black coloration. Mutations in kita, such as the well-characterized sparse mutant, lead to a severe reduction in melanophore number, establishing Kit as a key regulator of pigment cell differentiation and migration.\u003c\/p\u003e\n\u003cp\u003eThe Kit receptor is activated by its ligand, Kit ligand (Kitlg or Stem Cell Factor), which initiates downstream signaling through pathways such as MAPK\/ERK, PI3K\/Akt, and JAK\/STAT, promoting cell survival, proliferation, and migration. In addition to its role in pigmentation, zebrafish Kit signaling is implicated in the survival and migration of primordial germ cells (PGCs) and has been suggested to influence early blood cell development.\u003c\/p\u003e\n\u003cp\u003eZebrafish Kit proteins are functionally conserved with their mammalian counterparts, making the zebrafish model an effective system for investigating stem cell biology, oncogenic receptor tyrosine kinase signaling, and genetic disorders associated with KIT dysfunction, such as piebaldism and certain cancers.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Kita - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Kita - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Kita - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Kita - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Kita+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327326573,"sku":"RZ1184","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_12410548-5734-4b35-a3ea-0bee180b8d57.jpg?v=1772019453","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-kita-antibody-kit-scfr-bha17136379","provider":"BioHippo","version":"1.0","type":"link"}