{"product_id":"zebrafish-leo1-antibody-rna-polymerase-associated-protein-leo1-bha17136428","title":"Zebrafish Leo1 Antibody \/ RNA polymerase-associated protein LEO1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Leo1 Antibody \/ RNA polymerase-associated protein LEO1 is a anti-LEO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LEO1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLeo1 is a core component of the polymerase associated factor one complex, which is involved in the regulation of transcription elongation, chromatin modification, and gene expression. In zebrafish, Leo1 plays an important role in early development by controlling the expression of genes required for cell proliferation, differentiation, and organ formation.\u003cp\u003eThe Leo1 protein interacts with RNA polymerase two and other components of the transcription machinery to facilitate proper transcriptional progression and co transcriptional histone modifications. It is especially important in regulating genes involved in developmental pathways and stem cell maintenance.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish embryos, Leo1 is expressed in proliferative regions such as the developing brain, spinal cord, and somites. Functional studies have shown that disruption of Leo1 leads to defects in neural development, patterning of body structures, and cell cycle progression. These findings highlight its essential role in coordinating transcriptional programs during embryogenesis.\u003c\/p\u003e\n\u003cp\u003eBecause of its conservation across vertebrate species and its involvement in fundamental gene regulatory processes, zebrafish Leo1 is widely used in studies of transcriptional control, developmental biology, and epigenetic regulation. It is also a useful target for exploring how dysregulation of transcription elongation may contribute to developmental disorders and diseases such as cancer.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LEO1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LEO1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LEO1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LEO1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LEO1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047328637293,"sku":"RZ1233","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_10d9e2b2-5f94-4715-9299-3a3de3644862.jpg?v=1772019464","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-leo1-antibody-rna-polymerase-associated-protein-leo1-bha17136428","provider":"BioHippo","version":"1.0","type":"link"}