{"product_id":"zebrafish-myd88-antibody-bha17136437","title":"Zebrafish Myd88 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Myd88 Antibody is a anti-MYD88 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MYD88\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMyd88, or myeloid differentiation primary response protein 88, is an essential adaptor molecule in the innate immune system. In zebrafish, Myd88 mediates signal transduction from toll like receptors and interleukin one receptors, leading to activation of downstream inflammatory pathways that are critical for host defense.\u003cp\u003eMyd88 contains a death domain and a toll interleukin one receptor domain, which allow it to interact with both membrane bound receptors and cytoplasmic signaling proteins. Upon stimulation by pathogen associated molecular patterns such as bacterial lipopolysaccharide or viral RNA, toll like receptors recruit Myd88 to initiate signaling cascades that activate nuclear factor kappa B and mitogen activated protein kinases. This results in the production of proinflammatory cytokines and chemokines.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Myd88 is maternally deposited and expressed throughout development, with increased expression in immune tissues such as the kidney and intestine. It plays a vital role in early immune responses to infection and has been extensively used in zebrafish models of inflammation, pathogen challenge, and immune signaling.\u003c\/p\u003e\n\u003cp\u003eLoss of Myd88 function in zebrafish leads to impaired cytokine production and increased susceptibility to microbial infections, making it a valuable target for studies of innate immunity and inflammation. Due to its conservation across vertebrate species, zebrafish Myd88 is a widely used model for dissecting toll like receptor signaling and for testing immunomodulatory drugs.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MYD88 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MYD88 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MYD88 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MYD88 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MYD88+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329390957,"sku":"RZ1242","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d52017b7-f028-4dae-8001-387baf03d77c.jpg?v=1772019463","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-myd88-antibody-bha17136437","provider":"BioHippo","version":"1.0","type":"link"}