{"product_id":"zebrafish-nanos3-antibody-nanos-homolog-3-bha17136440","title":"Zebrafish Nanos3 Antibody \/ Nanos homolog 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Nanos3 Antibody \/ Nanos homolog 3 is a anti-NANOS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NANOS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNanos3 is a highly conserved RNA binding protein that plays an essential role in the development and maintenance of germ cells. In zebrafish, Nanos3 is required for the proper specification, migration, and survival of primordial germ cells during early embryogenesis.\u003cp\u003eZebrafish Nanos3 is an ortholog of the human NANOS3 gene. Both genes belong to the Nanos family of translational repressors and share conserved structural features, including zinc finger domains that enable binding to target messenger RNAs. Nanos3 acts primarily by interacting with other RNA binding proteins to repress the translation of specific transcripts involved in cell differentiation and apoptosis.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish embryos, Nanos3 is expressed maternally and zygotically in germ cell precursors. It ensures the correct localization and survival of these cells as they migrate toward the developing gonads. Loss of Nanos3 function in zebrafish leads to the mislocalization and eventual loss of germ cells, resulting in infertility or defective gonadal development.\u003c\/p\u003e\n\u003cp\u003eBecause of its conserved role in germline development, zebrafish Nanos3 serves as a valuable marker for early germ cells and is widely used in studies of reproductive biology, stem cell maintenance, and gene regulation. It is also a useful model for understanding human infertility and germ cell tumors.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NANOS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NANOS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NANOS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NANOS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NANOS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329489261,"sku":"RZ1245","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ae7b978f-e594-4413-ae51-1e5fd0cf325c.jpg?v=1772019465","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-nanos3-antibody-nanos-homolog-3-bha17136440","provider":"BioHippo","version":"1.0","type":"link"}