{"product_id":"zebrafish-ndr1-antibody-nodal-related-1-bha17136442","title":"Zebrafish Ndr1 Antibody \/ Nodal-related 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Ndr1 Antibody \/ Nodal-related 1 is a anti-NDR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNodal related 1, also known as Squint and Ndr1, is a member of the transforming growth factor beta superfamily and plays a key role in early embryonic development in zebrafish. It is involved in the establishment of the body axis, mesoderm and endoderm formation, and the specification of left right asymmetry. Nodal related 1 functions as a secreted signaling molecule that activates intracellular pathways through binding to type one and type two serine threonine kinase receptors.\u003cp\u003eZebrafish Nodal related 1 is considered a functional homolog of human NODAL, although it is not a direct one to one ortholog due to lineage specific gene duplications in teleost fish. However, both proteins are part of the same signaling pathway and perform highly conserved roles in vertebrate embryogenesis. Like human NODAL, zebrafish Nodal related 1 signals through the SMAD family of transcription factors and is regulated by feedback mechanisms involving antagonists such as Lefty and Cerberus.\u003c\/p\u003e\n\u003cp\u003eExpression of Nodal related 1 begins during the blastula stage and becomes concentrated in the embryonic margin, where it helps induce and pattern the mesendoderm. Mutations or knockdown of this gene result in severe defects in gastrulation, germ layer formation, and organ development. Due to its essential role in vertebrate development and strong functional conservation, zebrafish Nodal related 1 is widely studied in developmental biology, cell signaling, and disease modeling.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329554797,"sku":"RZ1247","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9a85dc55-a3df-4da0-b95f-3bb7019e9635.jpg?v=1772019465","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-ndr1-antibody-nodal-related-1-bha17136442","provider":"BioHippo","version":"1.0","type":"link"}