{"product_id":"zebrafish-ndr2-antibody-cyclops-nodal-related-2-precursor-bha17136443","title":"Zebrafish Ndr2 Antibody \/ Cyclops \/ Nodal-related 2 precursor","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Ndr2 Antibody \/ Cyclops \/ Nodal-related 2 precursor is a anti-NDR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDR2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNdr2, also called Nodal related protein 2 and Cyclops, is a secreted signaling molecule that belongs to the transforming growth factor beta superfamily. In zebrafish, Ndr2 plays an essential role in early embryonic development, particularly in the formation of mesoderm and endoderm, and in the establishment of the embryonic body axes. It functions through the Nodal signaling pathway, which regulates gene expression patterns necessary for germ layer differentiation and left right asymmetry.\u003cp\u003eZebrafish Ndr2 is closely related in function to the human NODAL protein. While it is not a direct one to one ortholog due to gene duplication events in zebrafish, Ndr2 and human NODAL share conserved domains and activate similar signaling cascades through type one and type two serine threonine kinase receptors and intracellular SMAD transcription factors. Both proteins are tightly regulated by extracellular antagonists such as Lefty and intracellular feedback loops.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish embryos, Ndr2 is expressed during gastrulation and is critical for the induction of the organizer region and patterning of the mesendoderm. Disruption of Ndr2 results in severe developmental defects, including failure to form the embryonic axis and internal organs. Because of its functional conservation with human NODAL and its central role in vertebrate development, zebrafish Ndr2 is widely used as a model to study signaling pathways involved in embryogenesis, tissue specification, and congenital malformations.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDR2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDR2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDR2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDR2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDR2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329128813,"sku":"RZ1248","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_49c6e24c-31fc-4a87-a26c-ad6f33aa632f.jpg?v=1772019464","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-ndr2-antibody-cyclops-nodal-related-2-precursor-bha17136443","provider":"BioHippo","version":"1.0","type":"link"}