{"product_id":"zebrafish-nkx2-2a-antibody-nkx2-2-bha17136445","title":"Zebrafish Nkx2.2a Antibody \/ Nkx2.2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Nkx2.2a Antibody \/ Nkx2.2 is a anti-Zebrafish Nkx2.2a Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Nkx2.2a\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNkx2.2a is a homeobox transcription factor that plays a critical role in the development of the central nervous system and pancreas in zebrafish. It belongs to the NK class of homeodomain proteins and functions by regulating gene expression involved in cell fate determination and patterning during embryogenesis.\u003cp\u003eZebrafish Nkx2.2a is one of two paralogs, the other being Nkx2.2b, resulting from a genome duplication event in teleosts. Nkx2.2a is considered a functional ortholog of the human NKX2 dash 2 gene. Both proteins are highly conserved and share similar developmental roles, particularly in the specification of ventral neural progenitors and in endocrine pancreas formation.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish embryos, Nkx2.2a is expressed in the ventral neural tube, including the regions that give rise to motor neurons, oligodendrocyte precursors, and serotonergic neurons. It acts downstream of sonic hedgehog signaling and is essential for defining the identity of specific neuronal subtypes. Nkx2.2a also contributes to the early development of the pancreas, where it helps regulate differentiation of insulin producing beta cells and other endocrine cell types.\u003c\/p\u003e\n\u003cp\u003eLoss of Nkx2.2a function in zebrafish leads to defects in neuronal patterning and impaired endocrine pancreas development, mirroring the phenotypes observed in mammalian systems. Due to its conservation with the human NKX2 dash 2 gene and its involvement in key developmental processes, zebrafish Nkx2.2a is widely used as a model to study neurogenesis, pancreas biology, and transcriptional regulation in vertebrates.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Nkx2.2a - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Nkx2.2a - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Nkx2.2a - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Nkx2.2a - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Nkx2.2a+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329358189,"sku":"RZ1250","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b78a5cdd-7e5d-40b0-8e92-ec68587eddd1.jpg?v=1772019464","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-nkx2-2a-antibody-nkx2-2-bha17136445","provider":"BioHippo","version":"1.0","type":"link"}