{"product_id":"zebrafish-noto-antibody-notochord-homeobox-protein-floating-head-bha17136446","title":"Zebrafish Noto Antibody \/ Notochord homeobox protein \/ Floating head","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Noto Antibody \/ Notochord homeobox protein \/ Floating head is a anti-Zebrafish Noto Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Noto\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNoto, or notochord homeobox protein, is a transcription factor that plays a fundamental role in the formation and patterning of the notochord and tailbud during early embryonic development. In zebrafish, Noto is essential for proper axis formation, neural tube development, and left right asymmetry.\u003cp\u003eZebrafish Noto is considered the functional ortholog of the human NOTO gene. Both zebrafish and human versions of this gene encode homeodomain containing transcription factors that are critical for notochord morphogenesis and cilia driven fluid flow, which influences organ laterality during development. Despite some sequence divergence between species, the developmental roles of zebrafish Noto and human NOTO are highly conserved.\u003c\/p\u003e\n\u003cp\u003eIn the zebrafish embryo, Noto is expressed in the dorsal organizer, the notochordal plate, and the developing tailbud. It regulates genes involved in axial mesoderm formation and ciliogenesis, and it interacts with other developmental signals including components of the nodal and hedgehog pathways. Loss of Noto function leads to defects in notochord structure, tail elongation, and laterality, including reversed heart and gut looping.\u003c\/p\u003e\n\u003cp\u003eBecause of its critical role in vertebrate axis and notochord formation, zebrafish Noto is widely used in developmental biology to study early mesodermal differentiation, organ positioning, and congenital disorders related to axis specification. Its functional conservation with the human NOTO gene also makes it a useful model for investigating the genetic basis of developmental syndromes affecting the spine and body plan.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Noto - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Noto - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Noto - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Noto - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Noto+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329653101,"sku":"RZ1251","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0208da8e-e8f9-4b0f-90e6-b5350baa454e.jpg?v=1772019464","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-noto-antibody-notochord-homeobox-protein-floating-head-bha17136446","provider":"BioHippo","version":"1.0","type":"link"}