{"product_id":"zebrafish-npas4l-antibody-cloche-neuronal-pas-domain-containing-protein-4-like-bha17136447","title":"Zebrafish Npas4l Antibody \/ Cloche \/ Neuronal PAS domain-containing protein 4-like","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Npas4l Antibody \/ Cloche \/ Neuronal PAS domain-containing protein 4-like is a anti-NPAS4L Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NPAS4L\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNpas4l, also known as neurogenic differentiation factor 4 like or cloche, is a basic helix loop helix PAS domain containing transcription factor that plays a critical role in early vascular and hematopoietic development in zebrafish. It functions as a master regulator in the specification of endothelial and blood progenitor cells during embryogenesis.\u003cp\u003eNpas4l is unique to zebrafish and closely related teleost species, and it does not have a confirmed one to one ortholog in humans. However, it is functionally analogous to members of the NPAS family of transcription factors in mammals, particularly those involved in neurogenesis and vascular development. Its closest functional equivalents in mammals are believed to include NPAS4 and potentially other early endothelial regulators, although no direct human counterpart fully recapitulates its role in vascular progenitor specification.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish embryos, Npas4l is expressed in the lateral plate mesoderm where it acts upstream of critical genes such as etsrp and tal1, which drive endothelial and hematopoietic differentiation. Loss of Npas4l results in the absence of blood and most vascular structures, making it essential for cardiovascular development.\u003c\/p\u003e\n\u003cp\u003eBecause of its pivotal and early role in blood and vessel formation, zebrafish Npas4l is a widely used genetic marker and model in studies of hematopoiesis, angiogenesis, and transcriptional regulation. It is especially valuable for dissecting the gene regulatory networks that control endothelial cell fate decisions in vertebrate embryos.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NPAS4L - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NPAS4L - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NPAS4L - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NPAS4L - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NPAS4L+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330111853,"sku":"RZ1252","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f4ce0364-9945-45d2-b73b-ff830cdd7194.jpg?v=1772019465","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-npas4l-antibody-cloche-neuronal-pas-domain-containing-protein-4-like-bha17136447","provider":"BioHippo","version":"1.0","type":"link"}