{"product_id":"zebrafish-nploc4-antibody-npl4-nuclear-protein-localization-protein-4-bha17136449","title":"Zebrafish Nploc4 Antibody \/ Npl4 \/ Nuclear protein localization protein 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Nploc4 Antibody \/ Npl4 \/ Nuclear protein localization protein 4 is a anti-NPLOC4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NPLOC4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNploc4, or nuclear protein localization protein four homolog, is a highly conserved protein involved in protein degradation and cellular stress response pathways. It acts as a cofactor in the disassembly of polyubiquitinated protein complexes, particularly in cooperation with the ATPase p97 also known as VCP. Nploc4 plays an essential role in extracting ubiquitinated substrates from membranes and protein complexes to facilitate their degradation by the proteasome.\u003cp\u003eIn zebrafish, Nploc4 is expressed in multiple tissues and is especially active in proliferative or metabolically active cells. The zebrafish Nploc4 protein is an ortholog of the human NPLOC4 gene. Both versions of the protein share strong sequence and functional conservation, indicating that zebrafish can serve as a reliable model for studying NPLOC4 related biology.\u003c\/p\u003e\n\u003cp\u003eFunctionally, Nploc4 is part of a complex with Ufd1 and p97, and this complex is critical for endoplasmic reticulum associated degradation, chromatin remodeling, and cell cycle regulation. Disruption of Nploc4 function in zebrafish leads to defects in protein homeostasis and developmental abnormalities, especially in systems that are sensitive to proteotoxic stress such as the nervous system and musculature.\u003c\/p\u003e\n\u003cp\u003eGiven its central role in maintaining cellular protein quality control and its conservation with human NPLOC4, zebrafish Nploc4 is commonly used in developmental studies and disease models related to protein aggregation, neurodegeneration, and cellular stress adaptation.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NPLOC4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NPLOC4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NPLOC4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NPLOC4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NPLOC4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329522029,"sku":"RZ1254","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f34f7420-1667-4b0e-8265-26ee8028c9a5.jpg?v=1772019466","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-nploc4-antibody-npl4-nuclear-protein-localization-protein-4-bha17136449","provider":"BioHippo","version":"1.0","type":"link"}