{"product_id":"zebrafish-p53-antibody-tp53-tumor-protein-53-bha17136454","title":"Zebrafish p53 Antibody \/ Tp53 \/ Tumor protein 53","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish p53 Antibody \/ Tp53 \/ Tumor protein 53 is a anti-P53 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e P53\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ep53, also known as Tumor Protein 53, is a crucial tumor suppressor protein that plays an essential role in maintaining cellular integrity by regulating the cell cycle, promoting DNA repair, and initiating apoptosis in response to stress, including DNA damage, hypoxia, and oxidative stress. It acts as a transcription factor and regulates the expression of a variety of target genes involved in these processes, thus preventing the accumulation of genetic mutations that could lead to cancer.\u003cp\u003eIn zebrafish, the p53 protein is an ortholog of the human TP53 gene, and both share highly conserved structural domains, including the DNA binding domain and the oligomerization domain, which are crucial for its function as a transcription factor. The zebrafish p53 protein shares many functional similarities with its human counterpart, including its ability to activate target genes like p21 and Bax in response to cellular stress and its involvement in apoptosis regulation. This makes zebrafish a valuable model for studying the role of p53 in cancer biology, DNA damage responses, and developmental processes.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish development, p53 is involved in embryogenesis, cell differentiation, and tissue remodeling. p53’s function is particularly important in the early stages of zebrafish development, where it helps maintain genomic stability by responding to various stress signals. In cases of severe DNA damage or other cellular insults, p53 activation can lead to cell cycle arrest or apoptosis, which ensures that damaged cells do not proliferate, thereby preventing potential tumor formation.\u003c\/p\u003e\n\u003cp\u003eThe zebrafish model has been widely used in cancer research due to the ease of genetic manipulation and the similarity of key signaling pathways like the p53 pathway. p53 mutations in zebrafish, like in humans, can lead to abnormal cellular proliferation, tissue development defects, and tumor formation. Understanding the molecular pathways controlled by p53 in zebrafish is crucial for modeling human diseases such as cancer, neurodegeneration, and aging.\u003c\/p\u003e\n\u003cp\u003eGiven its evolutionary conservation and critical role in cell cycle regulation, apoptosis, and tumor suppression, zebrafish p53 is a powerful tool for studying oncogenesis, genomic stability, drug screening, and genetic disorders in vertebrate models.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=P53 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=P53 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=P53 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/P53 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=P53+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329751405,"sku":"RZ1259","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_58cfd8ce-99d5-4495-ad63-5e5cdb5ee6aa.jpg?v=1772019468","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-p53-antibody-tp53-tumor-protein-53-bha17136454","provider":"BioHippo","version":"1.0","type":"link"}