{"product_id":"zebrafish-pabpc4-antibody-polyadenylate-binding-protein-bha17136456","title":"Zebrafish Pabpc4 Antibody \/ Polyadenylate-binding protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pabpc4 Antibody \/ Polyadenylate-binding protein is a anti-PABPC4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PABPC4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePabpc4 (Poly(A)-binding protein cytoplasmic 4) is a regulatory protein involved in RNA metabolism, particularly in the stabilization, translation, and decay of mRNA. It plays a central role in controlling the mRNA translation process and is essential for maintaining proper gene expression during cellular stress, development, and differentiation.\u003cp\u003eIn zebrafish, Pabpc4 is an ortholog of the human PABPC4 gene. Both zebrafish and human Pabpc4 share significant sequence similarity and conserved functional domains, particularly in the RNA-binding regions. This high level of conservation between zebrafish and human Pabpc4 makes the zebrafish protein an excellent model for studying RNA regulation and its impact on cellular processes like development, cell differentiation, and stress response.\u003c\/p\u003e\n\u003cp\u003eThe primary function of Pabpc4 in zebrafish is to bind to the poly(A) tail of mRNA, which is a key feature in regulating mRNA stability and translation initiation. Pabpc4 facilitates the recruitment of translation initiation factors to the mRNA and plays a role in translational control, particularly in response to environmental stressors or changes in nutrient availability. Pabpc4 also participates in mRNA decay pathways, ensuring that mRNAs are properly degraded when they are no longer needed, thus maintaining cellular homeostasis.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pabpc4 is expressed in multiple tissues, including the brain, muscle, and embryonic tissues, and is particularly important during early development. It regulates mRNA translation and is crucial for protein synthesis during the rapid cell divisions and differentiation events that occur during embryogenesis.\u003c\/p\u003e\n\u003cp\u003eGiven its role in RNA metabolism and the regulation of gene expression, zebrafish Pabpc4 is a valuable model for studying molecular biology, genetic regulation, and developmental processes. The conservation of its function across species also makes it relevant for research into human diseases related to RNA processing and gene regulation.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PABPC4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PABPC4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PABPC4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PABPC4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PABPC4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329816941,"sku":"RZ1261","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a2ab80ff-1300-4695-8eba-b3742677faaf.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pabpc4-antibody-polyadenylate-binding-protein-bha17136456","provider":"BioHippo","version":"1.0","type":"link"}