{"product_id":"zebrafish-pafah1b1-antibody-pafah1b1a-pafah1b1b-lissencephaly-1-bha17136457","title":"Zebrafish Pafah1b1 Antibody \/ Pafah1b1a \/ Pafah1b1b \/ Lissencephaly-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pafah1b1 Antibody \/ Pafah1b1a \/ Pafah1b1b \/ Lissencephaly-1 is a anti-PAFAH1B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAFAH1B1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePafah1b1 (Platelet-activating factor acetylhydrolase 1b subunit 1) is an enzyme that plays a crucial role in the metabolism of platelet-activating factor (PAF), a lipid mediator involved in inflammation, immune responses, and vascular function. Pafah1b1 is part of a larger enzyme complex that hydrolyzes the acetyl group from PAF, thereby modulating its biological activity and reducing its pro-inflammatory effects.\u003cp\u003eIn zebrafish, Pafah1b1 is an ortholog of the human PAFAH1B1 gene. Both zebrafish and human Pafah1b1 share a high degree of sequence conservation and functional similarity. This conservation makes zebrafish an excellent model for studying the biological functions of Pafah1b1 and its role in various cellular processes. Zebrafish Pafah1b1 is involved in the regulation of inflammatory responses, neurogenesis, and vascular development.\u003c\/p\u003e\n\u003cp\u003ePafah1b1 is primarily expressed in the brain, heart, and blood vessels during zebrafish development. It plays an essential role in the formation and maintenance of the vascular system, where it helps regulate vascular permeability and inflammation. In addition to its role in vascular biology, Pafah1b1 is important for neuronal development, particularly in neurogenesis and synaptic plasticity.\u003c\/p\u003e\n\u003cp\u003eThe zebrafish model is widely used to study developmental processes such as angiogenesis and neurogenesis, and the study of Pafah1b1 in zebrafish offers valuable insights into its functional roles in these pathways. Its involvement in immune modulation and neurodevelopmental diseases also makes zebrafish Pafah1b1 an important protein for modeling human conditions related to vascular diseases, neurological disorders, and inflammatory responses.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAFAH1B1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAFAH1B1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAFAH1B1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAFAH1B1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAFAH1B1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330275693,"sku":"RZ1262","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d2ff8d4d-a2e1-4e4a-9074-4a14aa3bfd46.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pafah1b1-antibody-pafah1b1a-pafah1b1b-lissencephaly-1-bha17136457","provider":"BioHippo","version":"1.0","type":"link"}