{"product_id":"zebrafish-pard3aa-antibody-partitioning-defective-3-bha17136458","title":"Zebrafish Pard3aa Antibody \/ Partitioning defective 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pard3aa Antibody \/ Partitioning defective 3 is a anti-PARD3AA Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm, cell membrane, cell junctions (human similarity).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PARD3AA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePard3aa (Partitioning defective 3 alpha) is a protein involved in regulating the polarization of cells during development and plays a critical role in establishing cellular asymmetry. It is a member of the Par protein family, which is essential for controlling the organization of the actin cytoskeleton, cell junctions, and cell signaling pathways. Pard3aa regulates processes like epithelial polarity, membrane trafficking, and cell-cell interactions, which are crucial for proper tissue development and function.\u003cp\u003eIn zebrafish, Pard3aa is an ortholog of the human PARD3 gene. Both zebrafish and human Pard3aa share high sequence similarity and functional conservation, particularly in the regulation of cell polarity and cellular architecture. The similarities between the zebrafish and human versions of Pard3aa make zebrafish an excellent model for studying the molecular mechanisms of cell polarity, embryonic development, and tissue morphogenesis.\u003c\/p\u003e\n\u003cp\u003ePard3aa plays a vital role in the development of epithelial tissues, including the brain, gut, and heart, where it helps to establish and maintain apical-basal polarity. During embryogenesis, Pard3aa is involved in controlling embryo axis formation, cell migration, and the establishment of cellular asymmetry. It regulates the distribution of key proteins involved in junction formation and membrane trafficking, ensuring proper cell-cell adhesion and tissue integrity.\u003c\/p\u003e\n\u003cp\u003eGiven its role in developmental biology, cellular architecture, and tissue polarity, zebrafish Pard3aa serves as a useful model for studying fundamental processes like neural development, organogenesis, and cell polarity diseases. It is also an important protein for investigating human conditions related to developmental disorders, epithelial cancers, and neurodegenerative diseases that involve defects in cell polarity and cellular organization.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PARD3AA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PARD3AA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PARD3AA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PARD3AA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PARD3AA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329948013,"sku":"RZ1263","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_166ce648-0bed-4566-8ab5-4ccf3f852afa.jpg?v=1772019470","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pard3aa-antibody-partitioning-defective-3-bha17136458","provider":"BioHippo","version":"1.0","type":"link"}