{"product_id":"zebrafish-pax6-antibody-pax6a-pax6b-bha17136395","title":"Zebrafish Pax6 Antibody \/ Pax6a \/ Pax6b","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pax6 Antibody \/ Pax6a \/ Pax6b is a anti-PAX6 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAX6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePaired box protein 6 (Pax6) is a highly conserved transcription factor that plays a central role in the development of the eye, central nervous system, and pancreas. In zebrafish (Danio rerio), Pax6 is encoded by two paralogous genes, pax6a and pax6b, both of which are essential for ocular morphogenesis, neurogenesis, and endocrine cell differentiation.\u003cp\u003eDuring embryogenesis, zebrafish Pax6 is expressed in the forebrain, optic vesicles, retina, and spinal cord, where it regulates the expression of genes critical for cell fate determination and tissue patterning. Pax6 is a key regulator of eye field specification and is required for the proper formation of structures such as the lens, retina, and optic tectum. In addition, it contributes to pancreatic islet cell development, particularly in the differentiation of insulin-producing Beta-cells.\u003c\/p\u003e\n\u003cp\u003eGiven its evolutionary conservation and well-characterized functions, zebrafish Pax6 is widely used as a model for studying ocular development, neural differentiation, congenital eye disorders (e.g., aniridia), and transcriptional control of organogenesis.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAX6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAX6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAX6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAX6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAX6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327916397,"sku":"RZ1200","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_17076d74-5c9c-492b-a3e1-234156c20c32.jpg?v=1772019455","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pax6-antibody-pax6a-pax6b-bha17136395","provider":"BioHippo","version":"1.0","type":"link"}