{"product_id":"zebrafish-pccb-antibody-propionyl-coa-carboxylase-beta-chain-bha17136459","title":"Zebrafish Pccb Antibody \/ Propionyl-CoA carboxylase beta chain","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pccb Antibody \/ Propionyl-CoA carboxylase beta chain is a anti-Zebrafish Pccb Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Pccb\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePccb (Propionyl-CoA carboxylase beta subunit) is a key enzyme involved in the propionate metabolism pathway, where it plays a crucial role in the conversion of propionyl-CoA to methylmalonyl-CoA, a step that is essential for the proper functioning of the citric acid cycle. Pccb is part of the larger propionyl-CoA carboxylase complex, which is responsible for maintaining metabolic homeostasis, particularly during the breakdown of certain amino acids and fatty acids.\u003cp\u003eIn zebrafish, Pccb is an ortholog of the human PCCB gene. Both zebrafish and human Pccb proteins share significant sequence conservation and functional similarity, especially in their roles in energy metabolism and biochemical processing. This conservation makes zebrafish an excellent model for studying the physiological and biochemical functions of Pccb, as well as its involvement in metabolic diseases.\u003c\/p\u003e\n\u003cp\u003eThe Pccb protein functions in the mitochondria, where it catalyzes the conversion of propionyl-CoA into methylmalonyl-CoA. This reaction is important for the metabolism of certain amino acids, particularly valine, isoleucine, and threonine, as well as for the catabolism of odd-chain fatty acids. Additionally, the proper function of Pccb is critical for the synthesis of succinyl-CoA, a key intermediate in the citric acid cycle, which is essential for energy production in cells.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Pccb is expressed in various tissues, with prominent expression in the liver, heart, and muscle, which are important for metabolic processing and energy regulation. The enzyme is especially critical during embryonic development, where metabolic pathways must be tightly regulated to support rapid growth and tissue differentiation.\u003c\/p\u003e\n\u003cp\u003eDefects in Pccb function in both zebrafish and humans can lead to methylmalonic acidemia, a disorder characterized by an accumulation of methylmalonic acid and propionyl-CoA in the blood, which can cause developmental delays, neurological impairment, and metabolic complications. Studying zebrafish Pccb offers valuable insights into the molecular basis of metabolic diseases and the role of propionate metabolism in maintaining cellular homeostasis.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Pccb - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Pccb - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Pccb - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Pccb - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Pccb+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330570605,"sku":"RZ1264","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_087cac1f-9054-49f6-a6a8-703594891c4e.jpg?v=1772019468","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pccb-antibody-propionyl-coa-carboxylase-beta-chain-bha17136459","provider":"BioHippo","version":"1.0","type":"link"}