{"product_id":"zebrafish-pcna-antibody-proliferating-cell-nuclear-antigen-bha17136460","title":"Zebrafish Pcna Antibody \/ Proliferating cell nuclear antigen","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pcna Antibody \/ Proliferating cell nuclear antigen is a anti-Zebrafish Pcna Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Pcna\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePcna (Proliferating Cell Nuclear Antigen) is a critical protein involved in DNA replication and repair. It functions as a clamp that encircles DNA, stabilizing the DNA polymerase during replication and facilitating the progression of the replication fork. Pcna also plays a key role in DNA damage response, cell cycle regulation, and chromatin remodeling, ensuring the integrity of the genome during cell division.\u003cp\u003eIn zebrafish, Pcna is an ortholog of the human PCNA gene. Both zebrafish and human Pcna proteins are highly conserved in terms of their sequence and function, with both being involved in regulating DNA replication and cell cycle progression. The zebrafish Pcna protein shares the same functional domains as the human protein, including the sliding clamp domain, which is essential for its role in DNA polymerase stabilization and in facilitating replication fork progression.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pcna is expressed in a variety of tissues, with prominent expression observed during embryonic development in rapidly proliferating cells. It is especially important in mitotic cells, where it helps facilitate efficient DNA replication during the S-phase of the cell cycle. In addition, Pcna participates in DNA repair pathways, including base excision repair and nucleotide excision repair, ensuring the correction of any replication errors or DNA damage caused by environmental stress.\u003c\/p\u003e\n\u003cp\u003eThe function of Pcna in zebrafish has been extensively studied, especially due to its role in development and tissue regeneration. During embryogenesis, Pcna is highly expressed in the neural tissue, heart, and muscle, which require constant cell division and DNA replication. Furthermore, Pcna is involved in the response to DNA damage and in the regulation of cell cycle checkpoints, especially in tissues undergoing rapid growth and development.\u003c\/p\u003e\n\u003cp\u003eGiven its central role in cellular proliferation, DNA repair, and genome stability, zebrafish Pcna is an essential protein for studying cell cycle regulation, DNA replication, mutagenesis, and genomic integrity. The high degree of conservation between zebrafish and human Pcna makes it an ideal model for investigating human diseases associated with cell cycle dysregulation, such as cancer and genetic disorders related to DNA repair.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Pcna - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Pcna - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Pcna - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Pcna - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Pcna+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329849709,"sku":"RZ1265","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_743fb975-81c9-4d78-931f-57b620a4cbeb.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pcna-antibody-proliferating-cell-nuclear-antigen-bha17136460","provider":"BioHippo","version":"1.0","type":"link"}