{"product_id":"zebrafish-pdx1-antibody-pancreas-duodenum-homeobox-protein-1-bha17136462","title":"Zebrafish Pdx1 Antibody \/ Pancreas\/duodenum homeobox protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pdx1 Antibody \/ Pancreas\/duodenum homeobox protein 1 is a anti-PDX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PDX1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePdx1 (Pancreatic and duodenal homeobox 1) is a transcription factor that plays a critical role in pancreatic development and insulin production. It is essential for the development of the pancreas and the maintenance of beta-cell function in adults. Pdx1 regulates the expression of genes involved in pancreatic morphogenesis, beta-cell differentiation, and glucose metabolism.\u003cp\u003eIn zebrafish, Pdx1 is an ortholog of the human PDX1 gene. Both zebrafish and human Pdx1 proteins share significant sequence similarity and functional conservation, particularly in regulating pancreatic development and insulin gene expression. The similarities between the zebrafish and human versions of Pdx1 make zebrafish an excellent model for studying pancreatic biology, beta-cell development, and diabetes.\u003c\/p\u003e\n\u003cp\u003eDuring embryogenesis, Pdx1 is highly expressed in the developing pancreas, where it directs the differentiation of pancreatic progenitor cells into insulin-producing beta cells. In zebrafish, Pdx1 is essential for the formation of the pancreatic islets and the regulation of insulin secretion in response to blood glucose levels. Pdx1 also plays a key role in beta-cell regeneration after injury, making it a critical factor in pancreatic plasticity.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pdx1 is expressed predominantly in the pancreas and duodenum during development and in adult stages. The protein regulates the expression of several downstream genes involved in pancreatic endocrine function and beta-cell maturation, including insulin, glucagon, and somatostatin. Pdx1 also interacts with other transcription factors involved in pancreatic development, such as Ngn3 and Pax4, to coordinate the formation of the pancreatic islet.\u003c\/p\u003e\n\u003cp\u003eGiven its essential role in pancreatic development, insulin production, and beta-cell regeneration, zebrafish Pdx1 is an important model for studying type 1 and type 2 diabetes, as well as pancreatic diseases and beta-cell dysfunction. The zebrafish model offers a unique opportunity to study pancreatic regeneration, gene regulation, and metabolic disorders in real time.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PDX1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PDX1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PDX1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PDX1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PDX1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331160429,"sku":"RZ1267","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b52914da-3de1-4972-86ee-7f34173eb7fe.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pdx1-antibody-pancreas-duodenum-homeobox-protein-1-bha17136462","provider":"BioHippo","version":"1.0","type":"link"}