{"product_id":"zebrafish-pgam1-antibody-pgam1a-pgam1b-phosphoglycerate-mutase-bha17136463","title":"Zebrafish Pgam1 Antibody \/ Pgam1a \/ Pgam1b \/ Phosphoglycerate mutase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pgam1 Antibody \/ Pgam1a \/ Pgam1b \/ Phosphoglycerate mutase is a anti-PGAM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PGAM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePgam1 (Phosphoglycerate mutase 1) is an enzyme involved in the glycolytic pathway, where it catalyzes the interconversion of 3-phosphoglycerate to 2-phosphoglycerate. This step is crucial for the energy metabolism of cells, as it facilitates the continuation of glycolysis and plays a vital role in cellular ATP production. Pgam1 is a phosphoglycerate mutase and is found in a variety of tissues, where it helps regulate cellular energy homeostasis and metabolic flexibility.\u003cp\u003eIn zebrafish, Pgam1 is an ortholog of the human PGAM1 gene. Both zebrafish and human Pgam1 proteins share significant sequence similarity and perform equivalent functions in glycolytic metabolism. The protein in both species is involved in the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate, ensuring the smooth progression of glycolysis. Zebrafish Pgam1 is widely expressed in tissues that rely on high levels of metabolic activity, including the muscle, brain, and liver.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pgam1 has isoforms, as is common for metabolic enzymes involved in energy metabolism. These isoforms may differ in their tissue distribution or functional properties, which is important for the regulation of glycolysis under different physiological conditions. These isoforms may be involved in tissue-specific functions and are essential for maintaining glycolytic flux in response to varying energy demands during development and growth.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Pgam1 plays a critical role during embryogenesis, where metabolic regulation is essential for proper growth and tissue differentiation. It is also important for muscle development and neural tissue formation, where metabolic demands are high. Pgam1 is expressed in developing tissues, particularly those undergoing rapid cell division and differentiation.\u003c\/p\u003e\n\u003cp\u003eGiven its role in metabolism, cellular energy regulation, and development, zebrafish Pgam1 is an important protein for studying glycolysis, energy metabolism, and diseases related to metabolic dysfunction. It is particularly valuable in studies of muscle metabolism, neurodevelopment, and embryonic development.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PGAM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PGAM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PGAM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PGAM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PGAM1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330308461,"sku":"RZ1268","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b798cebd-204d-46f1-88e2-7718998e79cb.jpg?v=1772019469","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pgam1-antibody-pgam1a-pgam1b-phosphoglycerate-mutase-bha17136463","provider":"BioHippo","version":"1.0","type":"link"}