{"product_id":"zebrafish-pi4kb-antibody-phosphatidylinositol-4-kinase-beta-bha17136465","title":"Zebrafish Pi4kb Antibody \/ Phosphatidylinositol 4-kinase beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pi4kb Antibody \/ Phosphatidylinositol 4-kinase beta is a anti-PI4KB Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm (ER, Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PI4KB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePi4kb (Phosphatidylinositol 4-kinase beta) is an enzyme involved in the phosphoinositide signaling pathway, playing a key role in the synthesis of phosphatidylinositol 4-phosphate (PI4P), a lipid molecule that serves as a precursor for other important phosphoinositides. Pi4kb is responsible for the phosphorylation of phosphatidylinositol at the 4-position of the inositol ring, which is a crucial step in regulating membrane trafficking, vesicular transport, and cell signaling. This enzyme is involved in numerous cellular processes, including endocytosis, vesicle formation, and membrane dynamics.\u003cp\u003eIn zebrafish, Pi4kb is an ortholog of the human PI4KB gene. Both zebrafish and human Pi4kb share substantial sequence conservation and functional similarities, particularly in their roles in lipid signaling and membrane trafficking. The high degree of conservation between species makes zebrafish an ideal model for studying the molecular function of Pi4kb in cellular signaling, membrane dynamics, and vesicular trafficking.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pi4kb is highly expressed in tissues with high demands for membrane turnover and vesicular trafficking, such as the brain, heart, and muscle. The protein plays a significant role in the formation and function of membrane-bound organelles, including endosomes, lysosomes, and the Golgi apparatus. It also regulates the recycling of phosphoinositides, which are essential for maintaining the integrity of cellular membranes and signal transduction pathways.\u003c\/p\u003e\n\u003cp\u003eThe Pi4kb gene in zebrafish has isoforms, which may differ in their tissue distribution and functional roles. These isoforms are thought to be important for the fine-tuned regulation of lipid signaling in different cell types and under varying physiological conditions. Isoform variation is likely linked to developmental stages and differentiation processes, particularly in tissues that require rapid changes in membrane composition.\u003c\/p\u003e\n\u003cp\u003eGiven its essential role in membrane dynamics, lipid signaling, and vesicular trafficking, zebrafish Pi4kb is an important model for studying diseases related to membrane dysfunction, neurological disorders, and metabolic diseases. Its role in regulating phosphoinositide metabolism also makes it a valuable target for research into diseases such as cancer, cardiovascular disorders, and neurodegenerative diseases.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PI4KB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PI4KB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PI4KB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PI4KB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PI4KB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329784173,"sku":"RZ1270","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2cba0eac-33b6-46f3-b931-dc98ada7a11b.jpg?v=1772019468","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pi4kb-antibody-phosphatidylinositol-4-kinase-beta-bha17136465","provider":"BioHippo","version":"1.0","type":"link"}