{"product_id":"zebrafish-polr2a-antibody-dna-directed-rna-polymerase-subunit-bha17136467","title":"Zebrafish Polr2a Antibody \/ DNA-directed RNA polymerase subunit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Polr2a Antibody \/ DNA-directed RNA polymerase subunit is a anti-POLR2A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLR2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePolr2a (RNA polymerase II subunit alpha) is a critical protein component of RNA polymerase II, the enzyme responsible for transcribing protein-coding genes into messenger RNA (mRNA) in eukaryotic cells. Polr2a is a core subunit of the RNA polymerase II complex, which plays a pivotal role in gene expression and transcription regulation. The protein is involved in the initiation, elongation, and termination of transcription, as well as the processing of nascent RNA transcripts.\u003cp\u003eIn zebrafish, Polr2a is an ortholog of the human POLR2A gene. The zebrafish and human Polr2a proteins are highly conserved, indicating a similar functional role in transcriptional regulation across species. Both proteins are integral parts of the RNA polymerase II complex, ensuring the synthesis of mRNA and playing a vital role in cellular gene expression.\u003c\/p\u003e\n\u003cp\u003ePolr2a is widely expressed across zebrafish tissues, including the brain, heart, and muscle, reflecting its essential function in general cellular processes and development. It is crucial for the transcription of protein-coding genes during early embryonic development, cell differentiation, and growth. The proper functioning of Polr2a is required for cell cycle progression, cellular maintenance, and response to environmental signals.\u003c\/p\u003e\n\u003cp\u003eZebrafish Polr2a has isoforms, which may exhibit variations in their expression patterns and functional properties. These isoforms could be related to developmental stages and tissue-specific functions, helping to fine-tune transcriptional regulation during embryogenesis and tissue-specific differentiation. The presence of isoforms may also suggest a level of functional specialization in response to cellular demands during development and environmental adaptation.\u003c\/p\u003e\n\u003cp\u003eGiven its role in transcription regulation, gene expression, and embryonic development, zebrafish Polr2a is a vital protein for studying gene regulation, transcriptional control, and developmental biology. It also provides a useful model for investigating transcriptional dysregulation in diseases such as cancer, neurodegenerative diseases, and genetic disorders related to gene expression abnormalities.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLR2A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLR2A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLR2A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLR2A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLR2A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330373997,"sku":"RZ1272","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_00983c06-9b30-4d02-9faf-89bba065f419.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-polr2a-antibody-dna-directed-rna-polymerase-subunit-bha17136467","provider":"BioHippo","version":"1.0","type":"link"}