{"product_id":"zebrafish-polr2b-antibody-dna-directed-rna-polymerase-subunit-beta-bha17136468","title":"Zebrafish Polr2b Antibody \/ DNA-directed RNA polymerase subunit beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Polr2b Antibody \/ DNA-directed RNA polymerase subunit beta is a anti-POLR2B Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLR2B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePolr2b (RNA polymerase II subunit beta) is a critical component of RNA polymerase II, the enzyme responsible for transcribing DNA into messenger RNA (mRNA) in eukaryotic cells. Polr2b is part of the RNA polymerase II complex, which is essential for the transcriptional regulation of protein-coding genes. As a subunit of RNA polymerase II, Polr2b is involved in transcription initiation, elongation, and termination, and it plays a vital role in maintaining genomic integrity and cellular function by ensuring the accurate transcription of genes.\u003cp\u003eIn zebrafish, Polr2b is an ortholog of the human POLR2B gene. The zebrafish and human Polr2b proteins exhibit significant sequence conservation, reflecting the evolutionary conservation of their functions in RNA transcription. This high degree of homology makes zebrafish an excellent model for studying the role of Polr2b in gene expression and transcription regulation across species.\u003c\/p\u003e\n\u003cp\u003ePolr2b is ubiquitously expressed in zebrafish tissues, with particularly high expression levels in tissues such as the brain, heart, and muscle. These tissues rely heavily on the proper regulation of gene expression during development and cellular differentiation. Polr2b is essential for the transcription of protein-coding genes during early embryogenesis, as well as throughout the life cycle of the organism, playing a key role in the regulation of cellular growth, differentiation, and organ development.\u003c\/p\u003e\n\u003cp\u003eZebrafish Polr2b has isoforms, which may vary in their functional properties and tissue-specific expression. These isoforms could be involved in the fine-tuned regulation of transcription during different developmental stages or in response to environmental cues. Isoform diversity in Polr2b allows the protein to meet the specific transcriptional needs of different cell types, especially in tissues undergoing rapid differentiation or experiencing specific environmental stressors.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLR2B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLR2B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLR2B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLR2B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLR2B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329915245,"sku":"RZ1273","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0b78e555-ec71-4b29-8c1f-ef85b9478f96.jpg?v=1772019468","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-polr2b-antibody-dna-directed-rna-polymerase-subunit-beta-bha17136468","provider":"BioHippo","version":"1.0","type":"link"}